Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily

Stefano Panno, Andrea Giovanni Caruso, Sofia Bertacca, Antonino Pisciotta, Rosario Di Lorenzo, Serafino Marchione, Slavica Matić and Salvatore Davino
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Stefano Panno: Department of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, Italy
Andrea Giovanni Caruso: Department of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, Italy
Sofia Bertacca: Department of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, Italy
Antonino Pisciotta: Department of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, Italy
Rosario Di Lorenzo: Department of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, Italy
Serafino Marchione: Osservatorio per le Malattie delle Piante di Palermo (OMP), Via Uditore 15, 90145 Palermo, Italy
Slavica Matić: Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), 10151 Turin, Italy
Salvatore Davino: Department of Agricultural, Food and Forest Sciences, University of Palermo, 90133 Palermo, Italy

Agriculture, 2021, vol. 11, issue 6, 1-16

Abstract: Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in four Sicilian provinces (Italy), and its genetic structure and molecular variability were studied. In detail, a total of 617 grapevine samples of 11 autochthonous grapevine cultivars were collected in 20 commercial vineyards. Preliminary screening by serological (DAS-ELISA) and molecular (RT-PCR) analyses for ArMV (arabis mosaic virus) and GFLV detection were conducted. Results obtained showed the absence of ArMV in all the samples analyzed, while 48 out of 617 samples gave positive results to GFLV, for a total of 9 out of 11 cultivars analyzed. Phylogenetic analyses carried out on the GFLV-CP gene of 18 Sicilian GFLV sequences selected in this study showed a certain degree of variability among the Sicilian isolates, suggesting a different origin, probably as a consequence of the continuous interchange of GFLV-infected propagating material with other Italian regions or viticultural areas located in other countries.

Keywords: GFLV; grapevine disease; molecular variability; DAS-ELISA; RT-PCR; phylogenetic analysis (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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