Enhanced Ruminal Fermentation Parameters and Altered Rumen Bacterial Community Composition by Formulated Rumen Buffer Agents Fed to Dairy Cows with a High-Concentrate Diet

Sonny C. Ramos, Chang-Dae Jeong, Lovelia L. Mamuad, Seon-Ho Kim, A-Rang Son, Michelle A. Miguel, Mahfuzul Islam, Yong-Il Cho and Sang-Suk Lee
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Sonny C. Ramos: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
Chang-Dae Jeong: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
Lovelia L. Mamuad: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
Seon-Ho Kim: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
A-Rang Son: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
Michelle A. Miguel: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
Mahfuzul Islam: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
Yong-Il Cho: Animal Disease and Diagnostic Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea
Sang-Suk Lee: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon 57922, Korea

Agriculture, 2021, vol. 11, issue 6, 1-20

Abstract: The effects of rumen buffer agents on ruminal fermentation parameters and bacterial community composition were determined using in vitro and in vivo experiments in three rumen-cannulated, high-concentrate fed Holstein Friesian dairy cows. Experiment 1 in vitro treatments included bentonite, calcium carbonate, calcium oxide, sodium bicarbonate, sodium sesquicarbonate, and processed coral, and unbuffered samples served as the control. Experiment 2 in vitro treatments were based on the formulation of various combinations of the buffer agents used in Experiment 1. Combinations were selected for the in vivo study based on their buffering ability. Calcium oxide, sodium bicarbonate, and sodium sesquicarbonate stabilized the ruminal pH and improved in vitro rumen fermentation. The combined buffer agents had a significant effect on pH, buffering capacity, total gas, and total volatile fatty acids. Firmicutes and Bacteroidetes were the dominant phyla in both treatments and the control. Ruminococcus and Prevotella were found to be the dominant genera. Ruminococcus bromii was predominant in the treatment group. Prevotella jejuni was more abundant in the control group compared to the treatment group, in which its abundance was very low. Ruminococcus flavefaciens and Intestinimonas butyriciproducens gradually increased in abundance as cows received treatment. Overall, a high-concentrate diet administered to cows induced adverse changes in ruminal pH; however, buffer supplementation enhanced ruminal fermentation characteristics and altered bacterial community, which could contribute to preventing ruminal acidosis.

Keywords: ruminal fermentation; bacteria community; Holstein Friesian cows; buffer agents (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
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