Prospects of Developing Novel Genetic Resources by Chemical and Physical Mutagenesis to Enlarge the Genetic Window in Bread Wheat Varieties

Momina Hussain, Maryyam Gul, Roop Kamal, Muhammad Atif Iqbal, Sana Zulfiqar, Ammad Abbas, Marion S. Röder, Quddoos H. Muqaddasi and Mehboob-ur-Rahman
Additional contact information
Momina Hussain: Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road Faisalabad, Faisalabad 38000, Pakistan
Maryyam Gul: Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road Faisalabad, Faisalabad 38000, Pakistan
Roop Kamal: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Stadt Seeland, OT Gatersleben, Germany
Muhammad Atif Iqbal: Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road Faisalabad, Faisalabad 38000, Pakistan
Sana Zulfiqar: Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road Faisalabad, Faisalabad 38000, Pakistan
Ammad Abbas: Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road Faisalabad, Faisalabad 38000, Pakistan
Marion S. Röder: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Stadt Seeland, OT Gatersleben, Germany
Quddoos H. Muqaddasi: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Stadt Seeland, OT Gatersleben, Germany
Mehboob-ur-Rahman: Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road Faisalabad, Faisalabad 38000, Pakistan

Agriculture, 2021, vol. 11, issue 7, 1-16

Abstract: Sustainable production and improved genetic gains can be achieved by broadening the genetic window of elite wheat germplasm. Here, we induced mutations in two spring wheat varieties, viz., NN-Gandum-1 (NN-1) and Punjab-11 (Pb-11), by exposing their seeds to ethyl methane sulfonate (EMS) and γ-rays, respectively. We characterized >3500 lines of each NN-1 and Pb-11 derived population in three consecutive generations, viz., M 5 , M 6 , and M 7 , for important traits, e.g., plant height, heading date, spike morphology and rust resistance. We observed significant genetic variation and correlations in both populations for all investigated traits. We observed differences in terms of number of mutants between NN-1 (22.76%) and Pb-11 (26.18%) which could be ascribed to the genotype-by-mutagen interaction. High broad-sense heritability ( H 2 ) estimates, that are vital for higher genetic gains, were observed for all of the investigated traits in both populations ( H 2 = 0.69–0.91 in NN-1 and 0.84–0.98 in Pb-11). Particularly, to breed for rust resistance, we selected a subset ( n = 239) of M 7 lines that also showed phenotypic variation for other traits. Our studies (1) show the relevance to artificial mutagenesis to create genetic variation in elite germplasm for their immediate use in current breeding programs, and (2) provide material for downstream identification of genes associated with traits of high agronomic importance.

Keywords: Triticum aestivum L.; EMS; gamma radiation; z-score; mutagen-effectiveness; heritability; genetic resources (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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