Using Simple Sequence Repeats in 9 Brassica Complex Species to Assess Hypertrophic Curd Induction

Simone Treccarichi, Cornelia Di Gaetano, Fulvio Di Stefano, Mauro Gasparini and Ferdinando Branca
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Simone Treccarichi: Food and Environment (Di3A), Department of Agriculture, University of Catania, 95131 Catania, Italy
Cornelia Di Gaetano: Department of Medical Science, University of Turin, 10126 Turin, Italy
Fulvio Di Stefano: Department of Mathematical Science, Politecnico di Torino, 10126 Turin, Italy
Mauro Gasparini: Department of Mathematical Science, Politecnico di Torino, 10126 Turin, Italy
Ferdinando Branca: Food and Environment (Di3A), Department of Agriculture, University of Catania, 95131 Catania, Italy

Agriculture, 2021, vol. 11, issue 7, 1-10

Abstract: Five Simple Sequence Repeats (SSRs) were used to assess the relationship between inflorescence characteristics and their allelic variation in 53 Brassica oleracea and Brassica wild relatives ( n = 9). Curd morphometric traits, such as weight (CW), height (CH), diameter (CD1), shape (CS) inflorescence curvature angle (CA), and its curd stem diameter (CD2), were measured. The aim of the work was to analyze the relationships among the allelic patterns of the SSRs primers utilized, and their status of homo or heterozygosity registered at each locus, as well as the inflorescence morphometric traits in order to individuate genomic regions stimulating the hypertrophy of this reproductive organ. The relationships found explain the diversity among B . oleracea complex species ( n = 9) for the inflorescence size and structure, allowing important time reduction during the breeding process by crossing wild species, transferring useful resistance, and organoleptic and nutraceutical traits. The five SSRs loci were BoABI1, BoAP1, BoPLD1, BoTHL1, and PBCGSSRBo39. According to the allelic variation ascertained, we evaluated the heterozygosity index (H) for each SSR above cited. The results showed a significant interaction between the H index of the BoPLD1 gene and the inflorescence characteristics, summarized by the First Principal Component (PC1) ( p -value = 0.0244); we ascertained a negative correlation between the H index and inflorescence characteristics, namely CW, CH, CD1, CD2, CA. The homozygosity BoPLD1 alelles, indicated by the H index, affect the inflorescence characteristics and broccoli and cauliflower yields.

Keywords: Brassica complex species; MADH–box genes; SSRs assay; heterozygosity index; allelic variance; curd morphometric traits (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
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