A Facile and Modified Scheme for Synchronization and Isolation of Nematode Eggs

Satish Kumar Rajasekharan, Chaitany Jayprakash Raorane and Jintae Lee
Additional contact information
Satish Kumar Rajasekharan: School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea
Chaitany Jayprakash Raorane: School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea
Jintae Lee: School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea

Agriculture, 2021, vol. 11, issue 7, 1-7

Abstract: Nematodes are common pests that damage agricultural crop plants. Some of them are beneficial while others are parasitic and harmful to plants, animals and humans. Several in vitro studies have aimed to develop chemicals to kill parasitic nematodes, while others have been conducted to use beneficial nematodes as biocontrol agents. However, the preparation of large quantities of nematode eggs in a laboratory setting is challenging. Traditional egg isolation protocols involve the use of sieves to filter eggs or the use of chemicals that can be harmful to nematodes while isolating the eggs. Our method utilizes 1.5 × 1.5 cm sized chunks of bacterial or fungal feed to lure nematodes. A subsequent gentle washing of the consumed chunk with distilled water provides a rapid and straightforward method of collecting eggs in 6-well polystyrene plates and removing unwanted nematodes. Approximately 4000 Bursaphelenchus xylophilus eggs from a fungal chunk and 2400 Caenorhabditis elegans eggs from a bacterial chunk were obtained when tested. This study shows a protocol for the isolation of eggs and synchronization of nematode stages that is relatively straightforward, rapid, eco-friendly, and efficient. The protocol also provides a chemical-free and a reliable, simple means of separating eggs from adults and induces the synchronization of nematodes based on the simple concept that gravid nematodes can be provoked to lay eggs by providing additional feed.

Keywords: eggs; Bursaphelenchus xylophilus; Caenorhabditis elegans; juveniles; nematode (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/11/7/676/pdf (application/pdf)
https://www.mdpi.com/2077-0472/11/7/676/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:11:y:2021:i:7:p:676-:d:596431

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().