Morphological, Cytological, and Molecular-Based Genetic Stability Analysis of In Vitro-Propagated Plants from Newly Induced Aneuploids in Caladium

Shuangying Yu, Xiaoqin Zhao, Yida Wang, Dongzhu Jiang, Yiming Zhang, Liu Hu, Yiqing Liu and Xiaodong Cai ()
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Shuangying Yu: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Xiaoqin Zhao: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Yida Wang: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Dongzhu Jiang: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Yiming Zhang: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Liu Hu: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Yiqing Liu: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
Xiaodong Cai: Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China

Agriculture, 2022, vol. 12, issue 10, 1-17

Abstract: Aneuploids are valuable materials of genetic diversity for genetic analysis and improvement in diverse plant species, which can be propagated mainly via in vitro culture methods. However, somaclonal variation is common in tissue culture-derived plants including euploid caladium. In the present study, the genetic stability of in vitro-propagated plants from the leaf cultures of two types of caladium ( Caladium × hortulanum Birdsey) aneuploids obtained previously was analyzed morphologically, cytologically, and molecularly. Out of the randomly selected 23 and 8 plants regenerated from the diploid aneuploid SVT9 (2 n = 2 x − 2 = 28) and the tetraploid aneuploid SVT14 (2 n = 4 x − 6 = 54), respectively, 5 plants from the SVT9 and 3 plants from the SVT14 exhibited morphological differences from their corresponding parent. Stomatal analysis indicated that both the SVT9-derived variants and the SVT14-originated plants showed significant differences in stomatal guard cell length and width. In addition, the variants from the SVT14 were observed to have rounder and thicker leaves with larger stomatal guard cells and significantly reduced stomatal density compared with the regenerants of the SVT9. Amongst the established plants from the SVT9, two morphological variants containing 3.14–3.58% less mean fluorescence intensity (MFI) lost one chromosome, and four variants containing 4.55–11.02% more MFI gained one or two chromosomes. As for the plants regenerated from the SVT14, one variant with significantly higher MFI gained two chromosomes and three plants having significantly lower MFI resulted in losing four chromosomes. Three, out of the twelve, simple sequence repeat (SSR) markers identified DNA band profile changes in four variants from the SVT9, whereas no polymorphism was detected among the SVT14 and its regenerants. These results indicated that a relatively high frequency of somaclonal variation occurred in the in vitro-propagated plants from caladium aneuploids, especially for the tetraploid aneuploid caladium. Newly produced aneuploid plants are highly valuable germplasm for future genetic improvement and research in caladium.

Keywords: chromosome loss or gain; flow cytometry analysis; somaclonal variation; SSR analysis (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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