Functional Interpretation of Cross-Talking Pathways with Emphasis on Amino Acid Metabolism in Rhizosphere Microbiome of the Wild Plant Moringa oleifera

Manal A. Tashkandi, Rewaa S. Jalal, Lina Baz, Mohammed Y. Refai, Ashwag Shami, Ruba Abdulrahman Ashy, Haneen W. Abuauf, Fatimah M. Alshehrei, Fawzia A. Alshubaily, Aminah A. Barqawi, Sahar Alshareef and Aala A. Abulfaraj ()
Additional contact information
Manal A. Tashkandi: Department of Biochemistry, College of Science, University of Jeddah, Jeddah 21493, Saudi Arabia
Rewaa S. Jalal: Department of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi Arabia
Lina Baz: Department of Biochemistry, Faculty of Science, King AbdulAziz University, Jeddah 21589, Saudi Arabia
Mohammed Y. Refai: Department of Biochemistry, College of Science, University of Jeddah, Jeddah 21493, Saudi Arabia
Ashwag Shami: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11617, Saudi Arabia
Ruba Abdulrahman Ashy: Department of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi Arabia
Haneen W. Abuauf: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 24381, Saudi Arabia
Fatimah M. Alshehrei: Department of Biology, Jumum College University, Umm Al-Qura University, P.O. Box 7388, Makkah 21955, Saudi Arabia
Fawzia A. Alshubaily: Department of Biochemistry, Faculty of Science, King AbdulAziz University, Jeddah 21589, Saudi Arabia
Aminah A. Barqawi: Department of Chemistry, Al-Leith University College, Umm Al Qura University, Makkah 28434, Saudi Arabia
Sahar Alshareef: Department of Biology, College of Science and Arts at Khulis, University of Jeddah, Jeddah 21921, Saudi Arabia
Aala A. Abulfaraj: Biological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia

Agriculture, 2022, vol. 12, issue 11, 1-22

Abstract: The functional processes and mutual benefits of the wild plant Moringa oleifera and its rhizosphere microbiome were studied via metagenomic whole-genome shotgun sequencing (mWGS) in comparison with a bulk soil microbiome. The results indicated high gene abundance of the four KEGG categories, “Cellular Processes”, “Environmental Information Processing”, “Genetic Information Processing”, and “Metabolism”, in the rhizosphere microbiome. Most of the enriched enzymes in rhizobacteria are assigned to the pathway “Amino acids metabolism”, where soil-dwelling microbes use amino acids as a defense mechanism against phytopathogens, while promoting growth, colonizing the cohabiting commensal microbes and conferring tolerance against abiotic stresses. In the present study, it was proven that these beneficial microbes include Bacillus subtilis , Pseudomonas fluorescens, and Escherichia coli . Mineral solubilization in these rhizobacteria can make nutrients available for plant utilization. These rhizobacteria extensively synthesize and metabolize amino acids at a high rate, which makes nitrogen available in different forms for plants and microbes. Amino acids in the rhizosphere might stand mainly as an intermediate switcher for the direction of the soil nitrogen cycle. Indole acetic acid (IAA) was proven to be synthesized by these beneficial rhizobacteria via route indole-3-pyruvate (IPyA) of the pathway “Tryptophan metabolism”. This hormone might stand as a shuttle signaling molecule between M. oleifera and its rhizobacteria. Tryptophan is also metabolized to promote other processes with important industrial applications. Rhizobacteria were also proven to breakdown starch and sucrose into glucose, which is the primary metabolic fuel of living organisms. In conclusion, we assume that the metabolic processes in the rhizosphere microbiome of this wild plant can be eventually utilized in boosting the sustainability of agriculture applications and the plant’s ability to benefit from soil nutrients when they are not in the form available for plant root absorption.

Keywords: mWGS; phytopathogen; rhizobacteria; solubilization; tryptophan; glucose (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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