Isolation, Characterization, and Identification of Zinc-Solubilizing Bacteria (ZSB) from Wetland Rice Fields in Peninsular Malaysia

Nur Maizatul Idayu Othman (), Radziah Othman, Ali Tan Kee Zuan, Aida Soraya Shamsuddin, Nur Badriyah Kamarul Zaman, Norazlina Abu Sari and Qurban Ali Panhwar
Additional contact information
Nur Maizatul Idayu Othman: Faculty of Plantation and Agrotechnology, Universiti Teknologi Mara (UiTM) Cawangan Melaka, Jasin Campus, Melaka 77300, Malaysia
Radziah Othman: Department of Land Management, Universiti Putra Malaysia, Serdang 43400, Malaysia
Ali Tan Kee Zuan: Department of Land Management, Universiti Putra Malaysia, Serdang 43400, Malaysia
Aida Soraya Shamsuddin: Department of Environmental and Occupational Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia
Nur Badriyah Kamarul Zaman: Faculty of Plantation and Agrotechnology, Universiti Teknologi Mara (UiTM) Cawangan Melaka, Jasin Campus, Melaka 77300, Malaysia
Norazlina Abu Sari: Faculty of Plantation and Agrotechnology, Universiti Teknologi Mara (UiTM) Cawangan Melaka, Jasin Campus, Melaka 77300, Malaysia
Qurban Ali Panhwar: Soil & Environmental Sciences Division, Nuclear Institute of Agriculture (NIA), Tandojam 70060, Pakistan

Agriculture, 2022, vol. 12, issue 11, 1-15

Abstract: Micronutrients, such as zinc (Zn), are essential for the growth and development of a wide range of crops. To overcome Zn deficiency in the soil, Zn-solubilizing bacteria (ZSB) have recently been employed. In the present study, samples from the rice fields in the state of Selangor, Malaysia, were collected to isolate, characterize, and identify the ZSB. A total of 88 strains were isolated, and only 9 strains were able to solubilize the insoluble Zn on zinc oxide (ZnO)-, zinc carbonate (ZnCO 3 )-, and zinc phosphate (Zn 3 (PO 4 ) 2 )-amended Tris-minimal media agar and broth assays. The highest Zn solubilization (20.99%) was measured for the TM23 isolate when exposed to Zn 3 (PO 4 ) 2 -modified media culture, whereas ZnCO 3 showed the lowest (3.35%) Zn solubilization by ZSB. In addition, nine isolated ZSB also exhibited plant-growth-promoting (PGP) traits, including nitrogen fixation ability, siderophore production, indole acetic acid production (35.28–65.48 mL −1 ), phosphate solubilization (27.69–77.38%), enzyme hydrolysis, and production of organic acids. Most of the isolated strains (88) were Gram-negative, except for TM54, which was Gram-positive. The four potential ZSB isolates based on 16RS rDNA sequence analysis were identified as Serratia sp. and Acinetobacter sp. Hence, this study’s findings suggest that these isolates could be prospective candidates to overcome Zn deficiencies and reduce the consumption of chemical fertilizers in agricultural areas.

Keywords: isolates; plant-growth-promoting traits; rice; solubilization; zinc deficiency (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/12/11/1823/pdf (application/pdf)
https://www.mdpi.com/2077-0472/12/11/1823/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:12:y:2022:i:11:p:1823-:d:960030

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().