ROS Regulate NCF2, Key Metabolic Enzymes and MDA Levels to Affect the Growth of Fusarium solani

Jie Li (), Lidan Feng, Dong Li, Xianglin Liu, Yangyang Pan, Jing He and Junxia Zhang
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Jie Li: College of Forestry, Gansu Agricultural University, Lanzhou 730070, China
Lidan Feng: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
Dong Li: College of Forestry, Gansu Agricultural University, Lanzhou 730070, China
Xianglin Liu: College of Forestry, Gansu Agricultural University, Lanzhou 730070, China
Yangyang Pan: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
Jing He: College of Forestry, Gansu Agricultural University, Lanzhou 730070, China
Junxia Zhang: College of Forestry, Gansu Agricultural University, Lanzhou 730070, China

Agriculture, 2022, vol. 12, issue 11, 1-14

Abstract: Fusarium solani is the most significant pathogen that causes root rot in wolfberry, which has led to serious economic losses in terms of production. As an important enzyme in organisms, NADPH oxidase produces ROS. However, the mechanism of ROS mediated by NADPH oxidase in the growth of F. solani has not been studied. In this study, F. solani colonies were treated with 40 µmol/L DPI and 0.0012% H 2 O 2 . The growth rate in terms of colonies, number of spores, key gene expression levels, activity of key enzymes and the content of key products of ROS metabolic pathways were determined. The results showed that the growth rate of colonies treated by DPI decreased by 19.43%, the number of macroconidia increased by 231.03%, the IOD/area values of O 2 − and H 2 O 2 decreased by 34.88% and 16.97%, respectively, the expression levels in terms of NCF2 , SOD1 , CTA1 and PXMP4 significantly decreased and the activities of SOD, CAT and POD decreased significantly, while the MDA content increased significantly. Additionally, in the case of the colonies treated with exogenous H 2 O 2 , the MDA content decreased significantly while the other indicators increased. Taken together, the NCF2 gene is involved in regulating the activity of NADPH oxidase and regulates the products of O 2 − and ROS metabolism enzyme genes and their activities to affect colony growth in the F. solani growth process.

Keywords: NADPH oxidase; ROS; Fusarium solani; growth rate; DPI (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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