Symbiotic and Antagonistic Functions of the Bacterium Burkholderia cepacia BsNLG8, from the Nilaparvata lugens (Stal)

Xuemei Wang, Rongrong Yang, Junaid Zafar, Chen Peng, Xuewei Zhang, Yingying Hong, Surajit De Mandal, Wenqing Zhang, Fengliang Jin and Xiaoxia Xu ()
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Xuemei Wang: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
Rongrong Yang: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
Junaid Zafar: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
Chen Peng: China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China
Xuewei Zhang: China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China
Yingying Hong: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
Surajit De Mandal: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
Wenqing Zhang: State Key Laboratory of Biocontrol to School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
Fengliang Jin: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
Xiaoxia Xu: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China

Agriculture, 2022, vol. 12, issue 12, 1-14

Abstract: Bacterial symbionts are widespread in insects and other animals. These microbes play crucial roles in many aspects of insect physiology and biology, including immunity, nutrition and confronting plant defenses. In the present study, we isolated and identified the bacterium Burkholderia cepacia BsNLG8 from the brown planthopper (BPHs), Nilaparvata lugens , a devastating pest of rice crops worldwide. Plate confrontation assay indicated that BsNLG8 significantly inhibited the growth of phytopathogenic fungi. In addition, the BsNLG8 strain demonstrated the ability to produce siderophores, which explains its antagonistic mechanism. Lastly, we explored the nicotine degradation ability of BsNLG8 using in vitro and in vivo methods. In vitro, HPLC analysis results showed that BsNLG8 could significantly reduce the concentration of nicotine in the medium at 36 h. Moreover, microinjection of BsNLG8 in axenic BPHs increased the survival rate of the host on nicotine-containing rice seedlings. These findings could serve as the basis of future research in deciphering the interaction between host and symbionts.

Keywords: Nilaparvata lugens (Stal); Burkholderia cepacia; antagonism; nicotine degradation; resistance (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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