Harnessing the Phytase Production Potential of Soil-Borne Fungi from Wastewater Irrigated Fields Based on Eco-Cultural Optimization under Shake Flask Method

Naila Sadaf, Muhammad Zulqurnain Haider, Naeem Iqbal, Muyassar H. Abualreesh and Aishah Alatawi
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Naila Sadaf: Department of Botany, Government College University, Faisalabad 38000, Pakistan
Muhammad Zulqurnain Haider: Department of Botany, Government College University, Faisalabad 38000, Pakistan
Naeem Iqbal: Department of Botany, Government College University, Faisalabad 38000, Pakistan
Muyassar H. Abualreesh: Department of Marine Biology, Faculty of Marine Sciences, King Abdualaziz University, P.O. Box 80207, Jeddah 21589, Saudi Arabia
Aishah Alatawi: Biology Department, Faculty of Science, University of Tabuk, Tabuk 71421, Saudi Arabia

Agriculture, 2022, vol. 12, issue 1, 1-15

Abstract: Indigenous fungi present in agricultural soils could have synchronized their inherent potentials to the local climatic conditions. Therefore, the fungi resident in the untreated wastewater irrigated agricultural field might develop their potential for producing various enzymes to handle the induced full organic load from domestic wastewater and toxic chemicals from the textile industry. Around 53 various fungal isolates were grown and separated from the soil samples from these sites through soil dilution, soil-culture plate, and soil-culture plate methods. All the purified fungi were subjected to a phosphatase production test, and only 13 fungal strains were selected as phosphatase producers. Among them, only five fungi identified as Aspergillus niger , Aspergillus flavus , Aspergillus fumigatus , Penicillium purourogenum, and Mucor rouxii based on morphological similarities, showing higher phosphate solubilizing indices, were utilized for eco-cultural fine-tuning to harness their full production potential under shake flask (SF) method. Among various media, orchestral tuning, 200 µM sodium phytate as substrate with 1.5 mL of inoculum size of the fungi, pH 7, temperature 30 °C, glucose, and ammonium nitrate as carbon and nitrogen additive with seven days of incubation were found to be the most appropriate cultural conditions to harness the phytase production potential of the selected fungi. Aspergillus niger and Aspergillus flavus showed initial phytase activity (5.2 Units/mL, 4.8 Units/mL) and phytase specific activity (2.85, 2.65 Units/mL per mg protein) during screening to be enhanced up to 17 ± 0.033 (Units/mL), 16 ± 0.033 (Units/mL) and (13 ± 0.012), 10 ± 0.066 (Units/mL per mg protein), respectively, with the above-mentioned conditions. The phytase enzyme produced from these fungi were found to be almost stable for a wide range of pH (4–8); temperature (20–60 °C); insensitive to Ca 2+ and Mg 2+ ions, and EDTA, Ni 2+ , and Ba 2+ inhibitors but highly sensitive to Mn 2+ , Cu 2+ , and Zn 2+ ions, and Co 2+ , Cr 3+ , Al 3+ , Fe 2+ and Ag 1+ inhibitors. It was suggested that both phytase-producing strains of A. niger and A. flavus or their crude phytase enzymes might be good candidates for application in soils to release phosphates from phytate and a possible valuable substitute of phosphate fertilizers.

Keywords: fungal phytase; eco-cultural optimization; soil born fungi; enzyme stability analysis; Aspergillus niger; Aspergillus flavus (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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