Genomic Instability in Somatic Hybridization between Poncirus and Citrus Species Aiming to Create New Rootstocks

Dominique Dambier, Pascal Barantin, Gabriel Boulard, Gilles Costantino, Pierre Mournet, Aude Perdereau, Raphaël Morillon and Patrick Ollitrault
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Dominique Dambier: CIRAD, UMR AGAP Institut, 34398 Montpellier, France
Pascal Barantin: CIRAD, UMR AGAP Institut, 34398 Montpellier, France
Gabriel Boulard: CIRAD, UMR AGAP Institut, 34398 Montpellier, France
Gilles Costantino: CIRAD, AGAP Institut, Institut Agro, INRAE, University Montpellier, 20230 San Giuliano, France
Pierre Mournet: CIRAD, UMR AGAP Institut, 34398 Montpellier, France
Aude Perdereau: Genoscope, Institut de Biologie François-Jacob, Commissariat à l’Energie Atomique (CEA), Université Paris-Saclay, 91000 Evry, France
Raphaël Morillon: CIRAD, UMR AGAP Institut, 34398 Montpellier, France
Patrick Ollitrault: CIRAD, UMR AGAP Institut, 34398 Montpellier, France

Agriculture, 2022, vol. 12, issue 2, 1-26

Abstract: Rootstocks are an important component for citrus adaptation to increasing biotic and abiotic stresses resulting from global climate change. There is a strong complementarity between Citrus species, which adapt to abiotic stresses, and Poncirus trifoliata and its intergeneric hybrids, which exhibit resistances or tolerances to major diseases and pests. Thus, symmetrical somatic hybridization between complementary diploid rootstocks of these two genera appears to be an efficient way to develop new tetraploid rootstocks in order to address the new challenges of the citrus industry. New intergeneric somatic hybrids were obtained by electrofusion between protoplasts of Citrus and P. trifoliata hybrids. Extensive characterization of the nuclear and cytoplasmic genomes was performed by genotyping-by-sequencing (GBS) analysis. This revealed diploid cybrids and nuclear somatic hybrids. Mitochondrial genomes were mostly inherited from the callus parent, but homologous recombination events were observed for one parental combination. Chloroplasts exhibited random uniparental inheritance. GBS revealed local chromosomal instabilities for all nuclear somatic hybrids and whole chromosome eliminations for two hybrids. However, at the whole genome level, symmetrical addition of the nuclear genomes of both parents was predominant and all somatic hybrids displayed at least one trifoliate orange haplotype throughout the genome.

Keywords: protoplast fusion; somatic embryogenesis; genotyping by sequencing; somaclonal variation; Citrus sinensis; Citrus reticulata; Poncirus trifoliata; citrange; citrumelo; citrandarin (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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