A High Sensitivity Electrochemical Immunosensor Based on Monoclonal Antibody Coupled Flower-Shaped Nano-ZnO for Detection of Tenuazonic Acid

Chi Zhang, Congcong Du, Wei Liu, Ting Guo, Ying Zhou, Hongyuan Zhou, Yuhao Zhang, Xiaozhu Liu and Liang Ma
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Chi Zhang: College of Food Science, Southwest University, Chongqing 400715, China
Congcong Du: College of Food Science, Southwest University, Chongqing 400715, China
Wei Liu: College of Food Science, Southwest University, Chongqing 400715, China
Ting Guo: College of Food Science, Southwest University, Chongqing 400715, China
Ying Zhou: College of Food Science, Southwest University, Chongqing 400715, China
Hongyuan Zhou: College of Food Science, Southwest University, Chongqing 400715, China
Yuhao Zhang: College of Food Science, Southwest University, Chongqing 400715, China
Xiaozhu Liu: Chongqing Micro Miracles Biotechnology Company, Chongqing 400039, China
Liang Ma: College of Food Science, Southwest University, Chongqing 400715, China

Agriculture, 2022, vol. 12, issue 2, 1-11

Abstract: In this paper, an electrochemical biosensor was established for the high-sensitivity detection of Tenuazonic acid (TeA) in fruits based on the enrichment of flower-shaped nano-ZnO and the specific recognition of immune response. Herein flower-shaped nano-ZnO (ZnO NFs) with a hexagonal wurtzite structure and diameter of 700–800 nm were demonstrated to have the optimal specific surface area and outstanding conductivity, compared with different morphology, sizes, and crystal structures of nano-ZnO. Second, the ZnO NFs were used as carriers for efficiently immobilizing monoclonal antibodies to obtain antibody bioconjugates, which were anchored on the 2-mercaptobenzoic acid-modified gold electrode by amide reaction. In the presence of TeA, the monoclonal antibody could specifically recognize and bind to it, resulting in a decrease in electron transfer ability on the gold electrode surface. Finally, the electrochemical biosensor showed a range from 5 × 10 −5 μg/mL to 5 × 10 −1 μg/mL with a detection limit of 1.14 × 10 −5 μg/mL. Furthermore, it exhibited high selectivity for TeA among other analogs, such as Altenuene (ALT) and Alternariol (AOH). Notably, the proposed strategy could be employed to monitor TeA in tomato and citrus, showing potential application prospects in practical application and commercial value.

Keywords: tenuazonic acid; flower-shaped nano-ZnO; monoclonal antibody; electrochemical immunosensor (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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