Comparative Transcriptome Analysis of Bt Resistant and Susceptible Strains in Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)

Yaling Lin, Qing Gao, Yueqin Wang, Zhenying Wang, Kanglai He, Suqin Shang and Tiantao Zhang
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Yaling Lin: Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China
Qing Gao: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yueqin Wang: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zhenying Wang: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Kanglai He: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Suqin Shang: Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China
Tiantao Zhang: State Key Laboratory for the Biology of the Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Agriculture, 2022, vol. 12, issue 2, 1-14

Abstract: The evolution of target pest population resistance to Bt toxins is the most relevant threat to the sustainability of Bt technology, thus it is necessary to clarify insect resistance mechanisms. Firstly, the resistance level of Asian corn borer was determined by bioassay. After 28 generations selection in the lab, the Cry1Ie-resistant strain (ACB-IeR) developed more than 862-fold resistance to Cry1Ie, and the Cry1F-resistant strain (ACB-FR) developed 961-fold resistance to Cry1F. The results show that long-term exposure to Bt toxins can lead to resistance. Then, we compared the differential expression genes (DEGs) of ACB-FR and ACB-IeR with susceptible strain (ACB-BtS), and analyzed GO function and KEGG pathway through transcriptome sequencing. The comparison showed that in Bt-resistant strains, many genes have a significant down-regulated trend. Several Bt-resistance candidate genes were differentially expressed in both resistant strains. Furthermore, the DEGs were verified by RT-qPCR and showed similar trend. These results provide candidate genes for further research on the Bt resistance mechanism.

Keywords: Ostrinia furnacalis; Bacillus thuringiensis; Cry1F; Cry1Ie; transcriptome; resistance (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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