N 2 O Emission and Nitrification/Denitrification Bacterial Communities in Upland Black Soil under Combined Effects of Early and Immediate Moisture

Lei Wang, Da-Cheng Hao, Sisi Fan, Hongtu Xie, Xuelian Bao, Zhongjun Jia and Lianfeng Wang
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Lei Wang: Liaoning Provincial Universities Key Laboratory of Environmental Science and Technology, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
Da-Cheng Hao: Liaoning Provincial Universities Key Laboratory of Environmental Science and Technology, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
Sisi Fan: Liaoning Provincial Universities Key Laboratory of Environmental Science and Technology, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
Hongtu Xie: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
Xuelian Bao: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
Zhongjun Jia: Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
Lianfeng Wang: Liaoning Provincial Universities Key Laboratory of Environmental Science and Technology, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China

Agriculture, 2022, vol. 12, issue 3, 1-22

Abstract: Soil moisture is the major factor influencing microbial properties and nitrous oxide (N 2 O) production. Agricultural soils can be probed under wetting, wet/dry alternating, and constant moisture conditions to evaluate the combined effects of early (previous) and immediate (current) moisture on N 2 O emission and nitrification/denitrification. In view of the water history of upland black soil, five moisture regimes comprising different antecedent and present water holding capacity (WHC) levels were set up in the microcosm study. The 20% WHC was adopted as the initial legacy moisture, while three immediate water statuses include constant WHC, dry-wet cycle, and incremental moisture. Quantitative PCR and 16S rRNA amplicon sequencing were used to assess the impact of current and previous moisture on the bacterial community composition and abundance of nitrification/denitrification genes (amoA, nirS, and nosZ); the soil physicochemical properties, and N 2 O emission were monitored. The N 2 O production and nitrifying-denitrifying microbial communities were influenced by the antecedent moisture and pattern of the dry-wet cycle. The nitrifying-denitrifying microbial communities, especially members of β-/γ-Proteobacteria, Bacteroidetes and Gemmatimonadetes, in black soil were important in explaining the variation of N 2 O production. The key taxonomic groups in response to the moisture alteration, e.g., Acidobacteria, Sphingobacteriia, Deltaproteobacteria, Methylobacterium, Gemmatimonas and Pseudarthrobacter, etc., were also highlighted. The soil nitrate, ammonium nitrogen, N 2 O emission, nitrification/denitrification and mineralization were profoundly impacted by water regimes and showed statistically significant correlation with specific bacterial genera; the nitrite/nitrate reduction to ammonium could be boosted by high moisture. Both nitrifier denitrification and heterotrophic denitrification could be enhanced substantially when the black soil moisture was increased to above 60% WHC. These findings help evaluate the effects of the water mode on the N 2 O emission from black soil, as well as the associated impacts on both soil fertility and the global environment.

Keywords: antecedent soil moisture; microbial community composition; nitrous oxide; gene abundance; MiSeq sequencing; legacy impact (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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