Genome-Wide Association Study Uncovers Loci and Candidate Genes Underlying Phytosterol Variation in Sesame ( Sesamum indicum L.)

Zhijian Wang, Qi Zhou, Senouwa Segla Koffi Dossou, Rong Zhou, Yingzhong Zhao, Wangyi Zhou, Yanxin Zhang, Donghua Li, Jun You and Linhai Wang
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Zhijian Wang: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Qi Zhou: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Senouwa Segla Koffi Dossou: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Rong Zhou: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Yingzhong Zhao: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Wangyi Zhou: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Yanxin Zhang: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Donghua Li: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Jun You: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Linhai Wang: Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China

Agriculture, 2022, vol. 12, issue 3, 1-13

Abstract: Sesame is one of the most important oilseed crops grown worldwide. It provides diverse nutraceuticals—including lignans, unsaturated fatty acids (UFA), phytosterols, etc.—to humans. Among sesame’s nutraceuticals, phytosterols have received less attention from sesame breeders, although their biological and pharmacological functions have been recorded. Therefore, in the present study, we evaluated the variation of phytosterol contents in 402 sesame accessions grown in two environments and revealed their associated loci and candidate genes. Gas chromatography (GC) analysis unveiled that sesame mainly contains four phytosterols: campesterol, stigmasterol, β-sitosterol, and Δ5-avenasterol. β-sitosterol (1.6–4.656 mg/g) was the major phytosterol, followed by campesterol (0–2.847 mg/g), stigmasterol (0.356–1.826 mg/g), and Δ5-avenasterol (0–1.307 mg/g). The total phytosterol content varied from 2.694 to 8.388 mg/g. Genome-wide association study identified 33 significant associated single nucleotide polymorphism (SNP) loci for the four traits, of which Ch6-39270 and Ch11-142842 were environmentally stable and simultaneously linked with campesterol and stigmasterol content variation. Candidate genes screening indicated that SINPZ1100015 encoding a NAC domain-containing protein 43 is likely the major candidate effect gene of phytosterol variation in sesame. The results of this study extend knowledge of phytosterol variation in sesame and provide important resources for markers-assisted breeding of high-phytosterol content varieties.

Keywords: sesame; plant sterols; GWAS; SNP loci; NAC protein; SiNST1 (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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