Cytpchrome P450 CYP4G68 Is Associated with Imidacloprid and Thiamethoxam Resistance in Field Whitefly, Bemisia tabaci (Hemiptera: Gennadius)

Jinjin Liang, Jing Yang, Jinyu Hu, Buli Fu, Peipan Gong, Tianhua Du, Hu Xue, Xuegao Wei, Shaonan Liu, Mingjiao Huang, Cheng Yin, Yao Ji, Chao He, Wen Xie, Ran Wang, Xin Yang and Youjun Zhang
Additional contact information
Jinjin Liang: College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
Jing Yang: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jinyu Hu: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Buli Fu: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Peipan Gong: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Tianhua Du: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Hu Xue: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xuegao Wei: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Shaonan Liu: College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
Mingjiao Huang: College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
Cheng Yin: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yao Ji: College of Plant Protection, Hunan Agricultural University, Changsha 410125, China
Chao He: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wen Xie: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Ran Wang: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
Xin Yang: Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Youjun Zhang: College of Plant Protection, Hunan Agricultural University, Changsha 410125, China

Agriculture, 2022, vol. 12, issue 4, 1-17

Abstract: The superfamily cytochrome P450s is involved in the evolution of insecticide resistance. However, whether CYP4G68 , a differentially expressed gene identified from our transcriptomics analysis, confers resistance to the world’s heavily used insecticide class neonicotinoids is unknown. Hence, we explored the role of CYP4G68 in conferring imidacloprid and thiamethoxam resistance in Bemisia tabaci . The species B. tabaci MED developed low-to-high resistance to imidacloprid and thiamethoxam. Exposure to imidacloprid and thiamethoxam significantly increased the expression of CYP4G68 . Moreover, quantitative real-time PCR analysis demonstrated that CYP4G68 was remarkably overexpressed in imidacloprid-resistant and thiamethoxam-resistant strains compared to susceptible strains. Further correlation analysis showed that CYP4G68 expression was significantly positively correlated with the associated resistance level in various strains of B. tabaci. These results suggest that the enhanced expression of CYP4G68 appears to mediate imidacloprid and thiamethoxam resistance in B. tabaci . Additionally, silencing CYP4G68 via RNA interference strongly increased the susceptibility of B. tabaci MED to imidacloprid and thiamethoxam. Collectively, this work revealed that CYP4G68 plays a vital role in imidacloprid and thiamethoxam resistance in B. tabaci MED. These findings will not only advance our understanding of the role of P450s in insecticide resistance but also provide a great potential target for the sustainable control of destructive insect pests such as whiteflies.

Keywords: cytochrome P450s; Bemisia tabaci; neonicotinoid resistance; sustainable pest control (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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