In Vitro Application of Exogenous Fibrolytic Enzymes from Trichoderma Spp. to Improve Feed Utilization by Ruminants

Francesco Iannaccone, Vittoria Alborino, Irene Dini, Anna Balestrieri, Roberta Marra, Rosario Davino, Antonio Di Francia, Felicia Masucci, Francesco Serrapica and Francesco Vinale
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Francesco Iannaccone: Department of Agricultural and Environmental Science, University of Bari “Aldo Moro”, 70126 Bary, Italy
Vittoria Alborino: Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Irene Dini: Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy
Anna Balestrieri: Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici, Italy
Roberta Marra: Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Rosario Davino: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80138 Naples, Italy
Antonio Di Francia: Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Felicia Masucci: Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Francesco Serrapica: Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
Francesco Vinale: BAT Center—Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055 Portici, Italy

Agriculture, 2022, vol. 12, issue 5, 1-16

Abstract: Treating ruminant feeds with exogenous fibrolytic enzymes may potentially increase forage cell wall degradability and thus feed efficiency. In nature, fungi biosynthesize lignocellulolytic enzymes that can break down lignocellulosic material into its sugar components, thereby providing ready fermentable substrates. This work showed the in vitro fibrolytic activity of three Trichoderma strains (T. atroviride strain P1, T. afroharzianum strain T22, T. reesei strain T67). Total protein concentration and enzymatic (e.g., glucanase, cellulase, and xylanase) activities were determined in fungal culture filtrates after 7 and 14 days of growth on different fiber-based media. The enzymatic mixtures produced by Trichoderma spp. showed the highest concentration of fibrolytic enzymes and were added to industrial feed to test their ability to hydrolyze insoluble fibers. The supplementation of industrial feeds containing medium-fiber or low-fiber concentrates with T22 enzymes produced in the presence of lyophilized mushrooms and durum wheat fiber reduced hemicellulose concentration up to 33% and 24%, respectively. These results may offer novel opportunities to develop livestock feeds with improved fiber digestibility.

Keywords: fibrolytic enzymes; Trichoderma; fiber degradability; cellulase activity; glucanase activity; xylanase activity (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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