Indirect Somatic Embryogenesis: An Efficient and Genetically Reliable Clonal Propagation System for Ananas comosus L. Merr. Hybrid “MD2”

Argelys Kessel-Domini, Daisy Pérez-Brito, Adolfo Guzmán-Antonio, Felipe A. Barredo-Pool, Javier O. Mijangos-Cortés, Lourdes Georgina Iglesias-Andreu, Alberto Cortés-Velázquez, Adriana Canto-Flick, Susana A. Avilés-Viñas, Yaritza Rodríguez-Llanes and Nancy Santana-Buzzy
Additional contact information
Argelys Kessel-Domini: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Daisy Pérez-Brito: GeMBio, Grupo de Estudios Moleculares Aplicados a la Biología, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Adolfo Guzmán-Antonio: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Felipe A. Barredo-Pool: Unidad de Biotecnología, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Javier O. Mijangos-Cortés: Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Lourdes Georgina Iglesias-Andreu: Instituto de Biotecnología y Ecología Aplicada, Campus para la Cultura, Universidad Veracruzana, las Artes y el Deporte. Av. de las Culturas Veracruzanas No. 101. Colonia Emiliano Zapata, Xalapa-Enríquez 91090, Mexico
Alberto Cortés-Velázquez: GeMBio, Grupo de Estudios Moleculares Aplicados a la Biología, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Adriana Canto-Flick: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Susana A. Avilés-Viñas: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Yaritza Rodríguez-Llanes: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico
Nancy Santana-Buzzy: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97205, Mexico

Agriculture, 2022, vol. 12, issue 5, 1-17

Abstract: The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple ( Ananas comosus L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants. Ten treatments, with different concentrations of 2,4-Dichlorophenoxyacetic (2,4-D) and Picloram (P), in the absence or presence of 6-Benzylaminopurine (BAP), were used for in vitro morphogenesis induction, as well as histological and molecular techniques, in order to characterize the morphogenic responses induced. Significant differences between treatments tested, to induce callus and buds, were assessed by the Kruskal Wallis method and the Mann–Whitney U-tests. Different pineapple regeneration routes were identified, showing the high regeneration potential of this species. The medium containing 2 mg L −1 2,4-D and 2 mg L −1 BAP, where indirect somatic embryogenesis occurred, was selected as the most efficient treatment, with an average of 120 somatic embryos per explant, differing significantly from the rest of the treatments. It was also demonstrated that the pineapple plants regenerated in vitro preserved the genetic identity of the donor genotype, which represents a high degree of confidence for the application of indirect somatic embryogenesis for A. comusus clonal propagation.

Keywords: Ananas comosus; somatic embryogenesis; micropropagation; organogenesis; SSR markers; ISSR markers; pineapple (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/12/5/713/pdf (application/pdf)
https://www.mdpi.com/2077-0472/12/5/713/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:12:y:2022:i:5:p:713-:d:818106

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().