Development of Multiple-Heading-Date mtl Haploid Inducer Lines in Rice

Jian Wang, Yuexuan Cao, Kejian Wang and Chaolei Liu
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Jian Wang: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
Yuexuan Cao: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
Kejian Wang: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
Chaolei Liu: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Agriculture, 2022, vol. 12, issue 6, 1-9

Abstract: In vivo doubled haploid (DH) production based on crossing heterozygous germplasm with mtl haploid inducer lines promises to transform modern rice ( Oryza sativa ) breeding. However, this technology is limited, as haploid inducers and pollen acceptors have asynchronous heading dates. To address this obstacle, we developed a panel of multiple-heading-date mtl haploid inducer lines that produce pollen for more than 35 days. We edited the MTL gene in a hybrid rice with the CRISPR-Cas9 system. We then selected transgene-free homozygous mutants in the T 1 generation and reproduced to T 4 generation by single-seed descent method. We obtained 547 mtl haploid inducers with diverse heading dates (from 73 to 110 days) and selected 16 lines comprising a core population with continuous flowering. The seed-setting rate and haploid induction rate (HIR) of the core panel were 4.0–12.7% and 2.8–12.0%, respectively. Thus, our strategy of using multiple-heading-date mtl haploid inducers could accelerate the use of in vivo DH technology in rice breeding.

Keywords: haploid inducers; MTL; multiple heading dates; rice; breeding; DH technology (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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