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Improved Production of Mashua ( Tropaeolum tuberosum ) Microtubers MAC-3 Morphotype in Liquid Medium Using Temporary Immersion System (TIS-RITA ® )

Gilmar Peña-Rojas, Roxana Carhuaz-Condori, Vidalina Andía-Ayme, Victor A. Leon and Oscar Herrera-Calderon
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Gilmar Peña-Rojas: Laboratory of Cellular and Molecular Biology, Biological Sciences Faculty, Universidad Nacional de San Cristóbal de Huamanga, Portal Independencia 57, Ayacucho 05003, Peru
Roxana Carhuaz-Condori: Laboratory of Cellular and Molecular Biology, Biological Sciences Faculty, Universidad Nacional de San Cristóbal de Huamanga, Portal Independencia 57, Ayacucho 05003, Peru
Vidalina Andía-Ayme: Laboratory of Food Microbiology, Biological Sciences Faculty, Universidad Nacional de San Cristóbal de Huamanga, Portal Independencia 57, Ayacucho 05003, Peru
Victor A. Leon: Department of Biology, New York University, New York, NY 10003, USA
Oscar Herrera-Calderon: Department of Pharmacology, Bromatology and Toxicology, Pharmacy and Biochemistry Faculty, Universidad Nacional Mayor de San Marcos, Lima 15001, Peru

Agriculture, 2022, vol. 12, issue 7, 1-7

Abstract: Essential molecules are embedded within the millenary crop Tropaeolum tuberosum (mashua); these compounds are critical for the Andean people’s traditional diet and extensively utilized by the pharmaceutical industry in Peru. In the Andean region, conventional cropping techniques generate microtubers susceptible to a viral infection, which substantially endangers mashua’s production. Therefore, we developed an innovative in vitro technique condition for enhancing the agriculture process for micro tubers production. The temporary immersion system (TIS) permits the production of high-quality microtubers in a reduced space, a lower amount of time, and in large quantities compared with tubers grown under traditional conditions. To obtain T. tuberosum ’s microtubers via TIS, we propagated seedlings, utilizing TIS-RITA ® vessels. A set of immersion frequency times were evaluated. Interestingly, results showed that immersion at 2 min every 3 h was more beneficial compared with 2 min every 5 h based on microtubers produced after 10 weeks from the treatments, revealing an efficient frequency setting which outputted improved microtubers quality and production.

Keywords: microtubers; temporary immersion system; Tropaeolum tuberosum; mashua (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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