Effects of Nutrients and Growth Regulators on Seed Germination and Development of Juvenile Rhizome Proliferation of Gastrodia elata In Vitro

Chi-Hung Hsieh, Zeng-Chin Liang (), Wen-Jang Shieh, Shin-Liang Chang and Wai-Jane Ho
Additional contact information
Chi-Hung Hsieh: Department of Medicinal Botanicals and Health Applications, College of Biotechnology and Bioresources, Da-Yeh University, Changhua 515006, Taiwan
Zeng-Chin Liang: Department of Medicinal Botanicals and Health Applications, College of Biotechnology and Bioresources, Da-Yeh University, Changhua 515006, Taiwan
Wen-Jang Shieh: Department of Medicinal Botanicals and Health Applications, College of Biotechnology and Bioresources, Da-Yeh University, Changhua 515006, Taiwan
Shin-Liang Chang: Department of Medicinal Botanicals and Health Applications, College of Biotechnology and Bioresources, Da-Yeh University, Changhua 515006, Taiwan
Wai-Jane Ho: Department of Medicinal Botanicals and Health Applications, College of Biotechnology and Bioresources, Da-Yeh University, Changhua 515006, Taiwan

Agriculture, 2022, vol. 12, issue 8, 1-22

Abstract: Two prevalent issues with Gastrodia elata growing in a natural wild environment are the necessary symbiotic seed germination with Mycena spp. and the long growing cycle of protocorms into mature rhizomes with Armillaria mellea . This study explored the most suitable nutrients to germinate seeds and the developmental stages and propagated protocorm/juvenile rhizomes (jrhs) through a tissue culture approach. Adding humic acid, mashed potato, peptone, and (indole-3-acetic acid) IAA to the 2 g/L Hyponex 7-6-19 basal medium accelerated seed germination into protocorms with an average length of 2 mm. The nutrients best for the in vitro propagation of jrhs consisted of 1 g/L Hyponex 7-6-19 and MS salts with 1/2 NH 4 NO 3 and KNO 3 , 2 mg/L BA, 2 mg/L NAA, 2 g/L tryptone, 2 g/L glutamic acid, 1 g/L chitosan, 10 g/L apple puree, 20 g/L mashed potato, 100 mL/L coconut water, and 1.5 g/L A. mellea powder, at a pH level of 6. The maximum length and number of jrhs were 17.2 ± 0.8 mm and 76.6, respectively, in two subcultures. The in vitro propagation system started from seeds of G. elata can be employed as a potential method to propagate protocorm/jrhs for the cultivation of mature rhizomes from indoor or outdoor cultures.

Keywords: Gastrodia elata; in vitro seed germination; protocorm development; juvenile rhizome multiplication; plant growth regulators; organic additives (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/12/8/1210/pdf (application/pdf)
https://www.mdpi.com/2077-0472/12/8/1210/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:12:y:2022:i:8:p:1210-:d:886934

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().