Incorporation of Two Bacterial Blight Resistance Genes into the Popular Rice Variety, Ranidhan through Marker-Assisted Breeding

Kartik Chandra Pradhan, Soumya Ranjan Barik, Shibani Mohapatra, Deepak Kumar Nayak, Elssa Pandit, Binod Kumar Jena, Sushree Sangeeta, Abhijit Pradhan, Abhishek Samal, Jitendiya Meher, Lambodar Behera, Debabrata Panigrahi, Arup Kumar Mukherjee and Sharat Kumar Pradhan ()
Additional contact information
Kartik Chandra Pradhan: College of Agriculture, OUAT, Bhubaneswar 751003, Odisha, India
Soumya Ranjan Barik: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Shibani Mohapatra: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Deepak Kumar Nayak: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Elssa Pandit: Department of Biosciences and Biotechnology, FM University, Balasore 756020, Odisha, India
Binod Kumar Jena: KVK, OUAT, Rayagada 765022, Odisha, India
Sushree Sangeeta: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Abhijit Pradhan: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Abhishek Samal: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Jitendiya Meher: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Lambodar Behera: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Debabrata Panigrahi: RRTTS, OUAT, Mahisapat, Denkanal 759013, Odisha, India
Arup Kumar Mukherjee: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India
Sharat Kumar Pradhan: ICAR-National Rice Research Institute, Cuttack 753006, Odisha, India

Agriculture, 2022, vol. 12, issue 9, 1-14

Abstract: The bacterial blight (BB) disease of rice is a major disease that reduces yield heavily in susceptible varieties. Ranidhan is a late maturing popular rice variety but shows high susceptibility to the disease. Two BB resistance genes were transferred into the variety through a marker-assisted backcross breeding approach. Tightly linked molecular markers were deployed to track the BB resistance genes in the plants carrying the target genes in each backcross generation. Foreground screening detected 17, 16 and 15 progenies to carry the 3 BB resistance genes in BC 1 F 1 , BC 2 F 1 and BC 3 F 1 generations, respectively. The selected BC 3 F 1 plant was selfed and three different combinations of BB resistance genes were tracked in homozygous state in seven BC 3 F 2 plants. The pyramided lines carrying three resistance genes in homozygous conditions were evaluated for BB disease resistance by inoculating with eight virulent Xoo strains. Five pyramided lines carrying two resistance gene combinations ( Xa21+xa13 and Xa21+xa5 ) exhibited enhanced resistance against the BB pathogens. The disease resistance was in the order of Xa21+xa5 < Xa21 + xa13 < xa13+xa5 gene combinations in conferring the resistance. The developed pyramided lines were similar to the recipient parent for the majority of the important agro-morphologic and grain quality traits.

Keywords: bacterial blight disease; foreground selection; resistance gene pyramiding; marker-assisted breeding; rice (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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