Heat Shock Treatment Promoted Callus Formation on Postharvest Sweet Potato by Adjusting Active Oxygen and Phenylpropanoid Metabolism

Qi Xin, Bangdi Liu (), Jing Sun, Xinguang Fan, Xiangxin Li, Lihua Jiang, Guangfei Hao, Haisheng Pei and Xinqun Zhou
Additional contact information
Qi Xin: Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Bangdi Liu: Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Jing Sun: Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Xinguang Fan: Key Laboratory of Agro-Products Primary Processing, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Xiangxin Li: Key Laboratory of Agro-Products Primary Processing, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Lihua Jiang: Key Laboratory of Agro-Products Primary Processing, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Guangfei Hao: Key Laboratory of Agro-Products Primary Processing, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Haisheng Pei: Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China
Xinqun Zhou: Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China

Agriculture, 2022, vol. 12, issue 9, 1-15

Abstract: This study aimed to investigate that rapid high-temperature treatment (RHT) at an appropriate temperature could accelerate callus formation by effectively promoting the necessary metabolic pathways in sweet potato callus. In this study, the callus of sweet potato was treated with heat shock at 50, 65, and 80 °C for 15 min. The callus formation was observed within 1, 3, and 5 days, and the accumulation of intermediates in the metabolism of phenylpropane and reactive oxygen species and changes in enzyme activities were determined. The results showed that appropriate RHT treatment at 65 °C stimulated the metabolism of reactive oxygen species at the injury site of sweet potato on the first day, and maintained a high level of reactive oxygen species production and scavenging within 5 days. The higher level of reactive oxygen species stimulated the phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase and cinnamate-4-hydroxylase activities of the phenylpropane metabolic pathway, and promoted the rapid synthesis of chlorogenic acid, p -coumaric acid, rutin, and caffeic acid at the injury site, which stacked to form callus. By Pearson’s correlation analysis, catalase (CAT), PAL, and chlorogenic acid content were found to be strongly positively correlated with changes in all metabolites and enzymatic activities. Our results indicated that appropriate high-temperature rapid treatment could promote sweet potato callus by inducing reactive oxygen species and phenylpropane metabolism; moreover, CAT, PAL, and chlorogenic acid were key factors in promoting two metabolic pathways in sweet potato callus.

Keywords: Rapid high temperature; Phenylalanine ammonia-lyase; Catalase; Chlorogenic acid; Key metabolic mechanisms (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.mdpi.com/2077-0472/12/9/1351/pdf (application/pdf)
https://www.mdpi.com/2077-0472/12/9/1351/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:12:y:2022:i:9:p:1351-:d:903532

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().