VvMYBPA2 Regulated the Accumulation of Flavan-3-ols though Forming a Trimeric Complex in ‘Zaoheibao’ Grape

Changmei Liang, Jianyong Guo, Mingxiang Chen, Xuehui Zhang, Guorong Zhang, Pengfei Zhang, Jinjun Liang and Pengfei Wen ()
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Changmei Liang: College of Information Science and Engineering, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Jianyong Guo: College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Mingxiang Chen: College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Xuehui Zhang: College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Guorong Zhang: College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Pengfei Zhang: College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Jinjun Liang: College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China
Pengfei Wen: College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China

Agriculture, 2022, vol. 12, issue 9, 1-12

Abstract: Flavan-3-ols are monomers of Proanthocyanidins (PAs), which are important polyphenolic compounds in grapes. Previous studies had shown that VvMYBPA2 was closely related to grape flavan-3-ol monomers biosynthesis, but its regulatory network is still unclear. Here, we found that the contents of (+)-catechin and (−)-epicatechin, the enzyme activities of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) and the expression of VvANR and VvLAR1 were increased in the VvMYBPA2 overexpression grape leaves compared to the control. It was proved that VvMYBPA2 protein interacted with VvWDR1 and VvWDR1 protein interacted with VvMYC2 by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). The promoters of VvANR and VvLAR1 were bound by VvMYBPA2 using yeast one-hybrid (Y1H) assay. These results suggested that VvMYBPA2 could form a trimeric complex with VvWDR1 and VvMYC2 and jointly regulated the expression of flavan-3-ol monomers related genes VvANR and VvLAR1 , thereby affecting the enzyme activities of ANR and LAR and ultimately regulating the contents of flavan-3-ols.

Keywords: grape; flavan-3-ols; proanthocyanidins; VvMYBPA2; MBW complex (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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