Effect of Sodium Selenite Concentration and Culture Time on Extracellular and Intracellular Metabolite Profiles of Epichloë sp. Isolated from Festuca sinensis in Liquid Culture

Lianyu Zhou (), Huichun Xie, Xuelan Ma, Jiasheng Ju, Qiaoyu Luo and Feng Qiao ()
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Lianyu Zhou: Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau, Xining 810008, China
Huichun Xie: Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau, Xining 810008, China
Xuelan Ma: Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau, Xining 810008, China
Jiasheng Ju: Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau, Xining 810008, China
Qiaoyu Luo: Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau, Xining 810008, China
Feng Qiao: Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau, Xining 810008, China

Agriculture, 2022, vol. 12, issue 9, 1-23

Abstract: Selenium (Se) is not only an essential trace element critical for the proper functioning of an organism, but it is also an abiotic stressor that affects an organism’s growth and metabolite profile. In this study, Epichloë sp. from Festuca sinensis was exposed to increasing concentrations of Na 2 SeO 3 (0, 0.1, and 0.2 mmol/L) in a liquid media for eight weeks. The mycelia and fermentation broth of Epichloë sp. were collected from four to eight weeks of cultivation. The mycelial biomass decreased in response to increased Se concentrations, and biomass accumulation peaked at week five. Using gas chromatography-mass spectrometry (GC-MS), approximately 157 and 197 metabolites were determined in the fermentation broth and mycelia, respectively. Diverse changes in extracellular and intracellular metabolites were observed in Epichloë sp. throughout the cultivation period in Se conditions. Some metabolites accumulated in the fermentation broth, while others decreased after different times of Se exposure compared to the control media. However, some metabolites were present at lower concentrations in the mycelia when cultivated with Se. The changes in metabolites under Se conditions were dynamic over the experimental period and were involved in amino acids, carbohydrates, organic acids, fatty acids, and nucleotides. Based on these results, we conclude that selenite concentrations and culture time influence the growth, extracellular and intracellular metabolite profiles of Epichloë sp. from F. sinensis.

Keywords: Epichloë sp. from Festuca sinensis; metabolomics; selenium; culture time (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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