Inhibition of the Growth and Development of Potato Early Blight Pathogen ( Alternaria solani ) by Combining Penicillium chrysogenum VKM F-4876D with Some Strobilurin-, Triazole-, and Phenylpyrrole-Based Fungicides

Amjad Hatem, Nataliya Karpova, Vera Yaderets, Elena Glagoleva, Kseniya Petrova, Alexandra Shibaeva, Alexander Ovchinnikov and Vakhtang Dzhavakhiya ()
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Amjad Hatem: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia
Nataliya Karpova: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia
Vera Yaderets: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia
Elena Glagoleva: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia
Kseniya Petrova: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia
Alexandra Shibaeva: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia
Alexander Ovchinnikov: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia
Vakhtang Dzhavakhiya: Laboratory of Biotechnology of Physiologically Active Compounds, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, pr-t 60-letiya Oktyabrya, 7, bld. 1, 117312 Moscow, Russia

Agriculture, 2022, vol. 12, issue 9, 1-19

Abstract: The biological control of fungal crop diseases based on the use of micro-organisms or their metabolites is a promising environmentally friendly alternative to common fungicide-based technologies. Penicillium fungi which synthesize various biologically active compounds with a wide range of antimicrobial activity are of special interest as potential producers of antifungal biopreparations. The purpose of this study was the evaluation of the antifungal activity of a dry biomass of the Penicillium chrysogenum VKPM F-4876D strain (DMP), both individually and combined with commercial azoxystrobin-, fludioxonil-, difenoconazole-, and tebuconazole-based fungicides commonly used to control early blight, towards Alternaria solani , an infectious agent of this potato disease. The study was performed under in vitro (cultivation on solid and liquid media) and in vivo (detached potato leaves) conditions. The cultivation of A. solani on agarized medium supplemented with 5 g/L of DMP caused significant morphological changes in pathogen colonies, whereas 7.5 g/L of DMP resulted in complete suppression of pathogen development. DMP addition to the liquid culture of A. solani significantly increased electrolyte release, i.e., impaired the cell membrane integrity in the pathogen. Combining DMP (1–2 g/L) with fungicides at a dose range of 0.01–10 mg/L resulted in significant in vitro growth inhibition of A. solani . The best result was obtained for the combination of DMP (1 g/L) with fludioxonil (1–5 mg/L) or tebuconazole (10 mg/L): a significant and prolonged antifungal effect (96–97% growth inhibition) was observed during the whole 14-day period of observation. The treatment of detached potato leaves with 1 g/L of DMP prior to artificial infection with A. solani resulted in a four-fold increase in the number of uninfected leaves and a two-fold reduction in the number of severely infected leaves compared to the control. These results demonstrate the possibility to develop an efficient environmentally friendly approach to manage potato early blight and provide prerequisites for the reduction in pesticide load on agrobiocoenoses, thus contributing to the restoration of ecological balance and the prevention of the emergence and spread of resistant pathogen strains.

Keywords: potato early blight; Alternaria solani; plant pathogens; Penicillium chrysogenum; dry biomass; antifungal activity (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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