Pseudomonas syringae Infection Modifies Chlorophyll Fluorescence in Nicotiana tabacum

Magdalena Tomaszewska-Sowa (), Norbert Keutgen, Tomáš Lošák, Anna Figas and Anna J. Keutgen
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Magdalena Tomaszewska-Sowa: Department of Agricultural Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, Poland
Norbert Keutgen: Department of Agricultural Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, Poland
Tomáš Lošák: Department of Environmentalistics and Natural Resources, Faculty of Regional Development and International Studies, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic
Anna Figas: Department of Agricultural Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029 Bydgoszcz, Poland
Anna J. Keutgen: Department of Crop Sciences, Institute of Vegetables and Ornamentals, University of Natural Resources and Life Sciences, Gregor Mendel Str. 33, 1180 Vienna, Austria

Agriculture, 2022, vol. 12, issue 9, 1-13

Abstract: The system Nicotiana tabacum L.— Pseudomonas syringae VAN HALL pv. tomato (Pto) DC3000 was investigated at a low inoculation level (c. 5 × 10 5 colony-forming units (CFU) mL –1 ) such as it occurs in the field. The aim of this study was to test the hypothesis that N. tabacum , a non-host of Pto DC3000, improved the PSII efficiency in inoculated leaves compared with control detached leaves. Visible symptoms at the infected area were not detected within 14 days. Chlorophyll (Chl) a fluorescence was measured 6–7 days after inoculation of detached leaves. Compared with the control, the actual photochemical quantum yield of photosystem (PS) II was higher in the inoculated leaves at the expense of the fraction of heat dissipated by photo-inactivated non-functional centers. In addition, the fraction of open PSII reaction centers (RCs) was higher in inoculated leaves. Maximum fluorescence in the dark-adapted detached inoculated leaves, as a measure of the absorbed energy, was lower than in control leaves. The lower capacity to absorb energy in combination with a higher fraction of open PSII RCs is interpreted as an acclimation to limit over-excitation and to reduce heat dissipation. This should limit the production of reactive oxygen species and reduce the probability of a hypersensitive response (HR), which represents an expensive cell-death program for the plant.

Keywords: Pseudomonas syringae VAN HALL pv. tomato DC3000; Nicotiana tabacum L.; steady state fluorometry; incompatible host plant—pathogen interaction; leaf detachment (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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