DDIT3 Governs Milk Production Traits by Targeting IL-6 to Induce Apoptosis in Dairy Cattle

Xiaogang Cui, Changqing Li, Zhangqi Wei, Hangting Meng, Fengfeng Zhang, Yue Liu, Changxin Wu and Shaohua Yang ()
Additional contact information
Xiaogang Cui: College of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei 230009, China
Changqing Li: College of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei 230009, China
Zhangqi Wei: College of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei 230009, China
Hangting Meng: Key Lab of Medical Molecular Cell Biology of Shanxi Province, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi Provincial Key Laboratory for Major Infectious Disease Response, Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China
Fengfeng Zhang: Key Lab of Medical Molecular Cell Biology of Shanxi Province, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi Provincial Key Laboratory for Major Infectious Disease Response, Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China
Yue Liu: Key Lab of Medical Molecular Cell Biology of Shanxi Province, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi Provincial Key Laboratory for Major Infectious Disease Response, Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China
Changxin Wu: Key Lab of Medical Molecular Cell Biology of Shanxi Province, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi Provincial Key Laboratory for Major Infectious Disease Response, Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China
Shaohua Yang: College of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei 230009, China

Agriculture, 2022, vol. 13, issue 1, 1-12

Abstract: The mechanisms of modulating milk production traits remain largely unknown. Based on our previous RNA-seq, DDIT3 was presumed as a novel, promising candidate gene for regulating milk protein and fat traits in dairy cattle. To further detect the genetic effect of DDIT3 and its potential molecular mechanisms in regulating milk production traits in dairy cattle, here, we performed a genotype-phenotype association study. Two SNPs, g.-1194 C>T and g.-128 C>T, were significantly associated with MY ( p = 0.0063), FY ( p = 0.0001) and PY ( p = 0.0216), respectively. A luciferase assay demonstrated that the allele T of g.-128 C>T increased the promoter activity by binding the HSF2, while allele C did not. To further reveal the molecular regulatory mechanisms, the DDIT3 -knockdown MAC-T cells were established. It was observed that DDIT3 silencing could induce apoptosis and increase the number of PI-positive cells. Meanwhile, DDIT3 silencing led to increased expression of inflammatory markers, such as IL-6 , IL6R , IL1B , IL7R , IL1RL2 , IL1A , STAT1-5 , MYC , IGFBP4, and IGFBP5 , and especially for IL-6 (log 2 FC = 4.22; p = 3.49 × 10 −112 ). Additionally, compared with the control group, increased lipid accumulation was found in the DDIT3 -knockdown MAC-T cells. Thus, our results proved that lower expression of DDIT3 could result in increased lipid accumulation and apoptosis via up-regulating the expression of IL-6. These findings provided clues about the regulatory mechanisms of milk production traits in dairy cattle.

Keywords: DDIT3; genetic effect; milk production traits; apoptosis; dairy cattle (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/13/1/117/pdf (application/pdf)
https://www.mdpi.com/2077-0472/13/1/117/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:13:y:2022:i:1:p:117-:d:1021764

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().