Does Sodium Nitroprusside Alleviate Water Deficit Stress in Impatiens walleriana Shoots Grown In Vitro?

Marija Đurić, Milana Trifunović-Momčilov (), Snežana Milošević, Marija Marković, Olga Radulović, Angelina Subotić and Branka Uzelac
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Marija Đurić: Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Milana Trifunović-Momčilov: Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Snežana Milošević: Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Marija Marković: Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Olga Radulović: Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Angelina Subotić: Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
Branka Uzelac: Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia

Agriculture, 2023, vol. 13, issue 10, 1-19

Abstract: Impatiens walleriana is a decorative horticultural plant species. Commercial production requires that the plants be brought to market, often accompanied by reduced water content during transport. Drought significantly affects metabolic processes in plants. The effects of polyethylene glycol (PEG)-induced water deficit on shoots of I. walleriana were investigated using different mechanisms at the biochemical level. In addition, the potential water deficit-ameliorating effect of sodium nitroprusside (SNP) was tested. Shoots of I. walleriana were initially grown on MS media supplemented with SNP (50, 100 and 250 μM). After pre-treatments, shoots were further transferred to media supplemented with PEG 8000 (3%) and/or SNP (50, 100 and 250 μM). Water deficit conditions increased proline, photosynthetic pigments, malondialdehyde (MDA), H 2 O 2 , total phenolic content and antioxidant activity. In addition, PEG-induced water deficit increased superoxide dismutase (SOD) and peroxidase (POX) activities but decreased catalase (CAT) activity. SNP did not significantly affect photosynthetic pigments and total phenolic content but increased proline accumulation, MDA and H 2 O 2 content, especially when applied simultaneously with PEG. Moreover, none of the investigated SNP pretreatments significantly altered the activities of SOD, POX, and CAT in I. walleriana . The results indicate that exogenous application of SNP effectively alleviated water deficit stress in shoots of I. walleriana grown in vitro.

Keywords: impatiens; drought stress; polyethylene glycol; sodim nitroprusside; reactive oxygen species; antioxidant enzymes; proline; polyphenols; photosynthetic pigments (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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