Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels

Ahmed Ateya (), Fatmah A. Safhi, Huda El-Emam, Marawan A. Marawan, Hayat Fayed, Amgad Kadah, Maha Mamdouh, Manar M. Hizam, Muath Q. Al-Ghadi, Mohamed Abdo, Liana Fericean, Rada Olga () and Ostan Mihaela
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Ahmed Ateya: Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Fatmah A. Safhi: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Huda El-Emam: Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Marawan A. Marawan: Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Benha 13511, Egypt
Hayat Fayed: Department of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Benha University, Benha 13511, Egypt
Amgad Kadah: Department of Physiology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt
Maha Mamdouh: Department of Physiology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt
Manar M. Hizam: College of Pharmacy, National University of Science and Technology, Nasiriyah 64001, Dhi Qar, Iraq
Muath Q. Al-Ghadi: Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Mohamed Abdo: Department of Animal Histology and Anatomy, School of Veterinary Medicine, Badr University in Cairo (BUC), Cairo 11829, Egypt
Liana Fericean: Department of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, Romania
Rada Olga: Department of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, Romania
Ostan Mihaela: Department of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, Romania

Agriculture, 2023, vol. 13, issue 10, 1-17

Abstract: The immune and antioxidant genetic factors that could converse with mastitis susceptibility in dromedary camels were looked at in this research. Of 120 female dromedary camels (60 healthy, and 60 with mastitis) were utilised. Each camel’s jugular vein was pierced to obtain five millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants to obtain whole blood and extract DNA and RNA. The immunological ( OTUD3 , TLR2 , TLR4 , STAB2 , MBL2 , TRAPPC9 , and C4A ) and antioxidant ( CAT , SOD3 , PRDX6 , OXSR1 , NDUFS6 , SERP2 , and ST1P1 ) genes’ nucleotide sequence polymorphisms between healthy and mastitis affected she-camels were discovered using PCR-DNA sequencing. Fisher’s exact test revealed that camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations propagating ( p < 0.01). Mastitic camels were significantly more likely to express the OTUD3 , TLR2 , TLR4 , STAB2 , MBL2 , TRAPPC9 , C4A , OXSR1 , SERP2 , and ST1P1 genes ( p < 0.05). However, CAT , SOD3 , PRDX6 , and NDUFS6 genes elicited a different pattern. The results may be used to develop management strategies and support the significance of nucleotide differences and gene expression patterns in these markers as indicators of the incidence of mastitis.

Keywords: immunity; antioxidant biomarkers; transcript levels; mastitis; dromedary camels (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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