A Novel PCR-Based Functional Marker of Rice Blast Resistance Gene Pi25

Yeyang Fan, Zhenhua Zhang, Derun Huang, Tingxu Huang, Hongfei Wang, Jieyun Zhuang and Yujun Zhu ()
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Yeyang Fan: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Zhenhua Zhang: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Derun Huang: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Tingxu Huang: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China
Hongfei Wang: Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China
Jieyun Zhuang: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China
Yujun Zhu: State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China

Agriculture, 2023, vol. 13, issue 10, 1-12

Abstract: Rice blast is arguably the most devastating fungal disease of rice. Utilization of resistance genes to breed resistant cultivars is one of the most economical and environmentally friendly approaches to combat the disease. Pi25 , a major resistance gene conferring broad-spectrum resistance to both leaf and neck blast, is an ideal gene resource to improve the resistance of rice varieties to blast. Recently, several allele-specific markers were developed. However, they were deficiently efficient due to either an additional process of restriction enzyme digestion for cleaved amplified polymorphic sequence (CAPS) markers or the risk of false-positive error in identifying susceptible Tetep allele ( Pi25 TTP ) for PCR-based markers. In this study, based on a conserved single nucleotide polymorphism (SNP) between resistant and susceptible alleles, a tetra-primer amplification refractory mutation system (ARMS)-PCR marker was developed. The new marker, namely Pi25-2687R3, could effectively distinguish the resistant Gumei 2 (GM2) allele ( Pi25 GM2 ) and the susceptible allele Pi25 TTP . Moreover, a perfect consistency of genotyping was exhibited between Pi25-2687R3 and published CAPS marker CAP3/ Hpy 99I. A more accurate genotyping was also displayed compared to the previous PCR-based SNP marker Pi25-2566. Our finding proved that Pi25-2687R3 could achieve the same result as CAP3/ Hpy 99I with less workload and cost and could promote the accuracy in the identification of genotypes superior to Pi25-2566. This study provided a quick and reliable functional marker for discriminating Pi25 alleles, which would be a valuable tool for genotypic assay and rice molecular breeding of blast resistance.

Keywords: rice blast; allele-specific marker; allele; tetra-primer amplification refractory mutation system (ARMS)-PCR; single nucleotide polymorphism; marker-assisted selection (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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