Identification of a Novel KTi-1 Allele Associated with Reduced Trypsin Inhibitor Activity in Soybean Accessions

Aron Park, Se-Hee Kang, Byeong-Hee Kang, Sreeparna Chowdhury, Seo-Young Shin, Won-Ho Lee, Jeong-Dong Lee, Sungwoo Lee, Yu-Mi Choi () and Bo-Keun Ha ()
Additional contact information
Aron Park: Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea
Se-Hee Kang: Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea
Byeong-Hee Kang: Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea
Sreeparna Chowdhury: Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea
Seo-Young Shin: Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea
Won-Ho Lee: Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea
Jeong-Dong Lee: Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
Sungwoo Lee: Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Republic of Korea
Yu-Mi Choi: National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Republic of Korea
Bo-Keun Ha: Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea

Agriculture, 2023, vol. 13, issue 11, 1-14

Abstract: Trypsin inhibitors (TIs) in soybean seeds reduce the availability of processed soybean foods and animal feed. This study aimed to evaluate the trypsin inhibitor activity (TIA) in 999 Korean soybean accessions and conduct molecular characterization of soybean accessions with low TIA. TIA was evaluated using colorimetric analysis through a substrate–enzyme reaction. The average TIA of the 999 soybean accessions was 90.31%, ranging from 43.70% to 99.51%. Kunitz trypsin inhibitor 3 (KTi-3) gene target sequencing analysis was performed on seven soybean germplasm accessions (IT105782, IT170889, IT273590, IT274513, IT274515, IT276197, and IT022891) showing less than 60% TIA. Four soybean accessions (IT274513, IT274515, IT276197, and IT022891) exhibited the same mutations (a G-to-T transversion and an AG deletion) in the KTi-3 gene ( Glyma.08g341500 ) in PI542044. In addition, whole-genome re-sequencing was performed on three soybean accessions with no mutations in the KTi-3 gene. Compared with the reference soybean genome, an in-frame insertion and five missense mutations were identified in the coding sequencing of the KTi-1 gene ( Glyma.01g09500 ) in IT105782. A RT-qPCR analysis showed that the mRNA expression level of KTi-1 was reduced by approximately 17% in IT105782 during seed development. In this study, we identified a previously unreported mutation in the KTi-1 gene and developed a KASP marker using this new KTi-1 variant.

Keywords: soybean; trypsin inhibitor (TI); trypsin inhibitor activity (TIA); KASP marker (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/13/11/2070/pdf (application/pdf)
https://www.mdpi.com/2077-0472/13/11/2070/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:13:y:2023:i:11:p:2070-:d:1269281

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().