Analyzing Single and Combined Cultures of Plant Growth-Promoting Rhizobacteria Isolates from Afghanistan as a Potential Biofertilizer for Rice Growth and Development

Safiullah Habibi, Tadashi Yokoyama, Mohammad Daud Haidari, Akihiro Torii, Michiko Yasuda and Naoko Ohkama-Ohtsu ()
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Safiullah Habibi: Faculty of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Saiwai-cho 3-5-8, Fuchu-shi 183-8509, Tokyo, Japan
Tadashi Yokoyama: Institute of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Saiwai-cho 3-5-8, Fuchu-shi 183-8509, Tokyo, Japan
Mohammad Daud Haidari: Faculty of Agriculture, Kabul University, Kart-e-Char, Kabul 1006, Afghanistan
Akihiro Torii: Faculty of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Saiwai-cho 3-5-8, Fuchu-shi 183-8509, Tokyo, Japan
Michiko Yasuda: Institute of Global Innovation Research, Tokyo University of Agriculture and Technology (TUAT), Harumi-cho 3-8-1, Fuchu-shi 183-8509, Tokyo, Japan
Naoko Ohkama-Ohtsu: Faculty of Agriculture, Kabul University, Kart-e-Char, Kabul 1006, Afghanistan

Agriculture, 2023, vol. 13, issue 12, 1-14

Abstract: Developing multi-functional, environmentally friendly biofertilizers can help reduce chemical fertilizer usage. This study examined single and combined application effects of four plant growth-promoting rhizobacteria (PGPR) strains: AF124 ( Rhizobium daejeonense ), AF74 ( Enterobacter cloacae ), AF96 ( Pseudomonas putida ), and AF42 ( Enterobacter cloacae ), isolated from alkaline salt-stressed soils from Afghanistan. Physiological features such as nitrogen (N 2 ) fixation, indole-3-acetic acid (IAA), organic acid production, and P and K solubilization of bacteria in single or combined cultures in vitro were examined. A single application of AF124 and AF74 showed significantly higher IAA production than the combined application of dual, triple, and quadruple PGPR treatments. Regarding acetylene reduction activity, an N 2 -fixation activity indicator, a single application of AF124 showed the highest value, followed by a triple application of AF124 + AF96 + AF42. The P-solubilization activity and organic acid production were lower in the single application of AF124 but higher with other strains in the combined applications, which resulted in the secretion of multiple organic acids into the medium. The highest solubilized K value was obtained from the quadruple application compared to other treatments. In the rice inoculation test, all single and combined PGPR treatments increased its growth parameters, such as root length and root and shoot dry biomass. The present results indicate that these four PGPR strains can potentially contribute to developing multi-functional bio-fertilizer formulations.

Keywords: PGPR; rice; N 2 -fixation; IAA production; nutrient solubilization (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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