Potential Anti-Sarcopenia Effect and Physicochemical and Functional Properties of Rice Protein Hydrolysate Prepared through High-Pressure Processing

Shih-Hsiao Chen, Po-Hsien Li (), Yung-Jia Chan, Yu-Tsung Cheng, Hui-Yao Lin, Shih-Chieh Lee, Wen-Chien Lu, Yu-Xu Ma, Min-Ying Li and Tuzz-Ying Song ()
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Shih-Hsiao Chen: Department of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, 168, Xuefu Road, Dacun Township, Changhua City 515006, Taiwan
Po-Hsien Li: Department of Food and Nutrition, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung City 43301, Taiwan
Yung-Jia Chan: College of Biotechnology and Bioresources, Da-Yeh University, 168, University Road, Dacun, Changhua City 515006, Taiwan
Yu-Tsung Cheng: Cardiovascular Center, Taichung Veterans General Hospital, 1650 Sec. 4 Taiwan Boulevard, Xitun Dist., Taichung City 40705, Taiwan
Hui-Yao Lin: Mirthful Biotech Co., Ltd., No. 23, Ln. 85, Yongfeng Road, Taiping Dist., Taichung City 41165, Taiwan
Shih-Chieh Lee: Department of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, 168, Xuefu Road, Dacun Township, Changhua City 515006, Taiwan
Wen-Chien Lu: Department of Food and Beverage Management, Chung-Jen Junior College of Nursing, Health Sciences and Management, 217, Hung-Mao-Pi, Chia-Yi City 60077, Taiwan
Yu-Xu Ma: Department of Food and Nutrition, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung City 43301, Taiwan
Min-Ying Li: Department of Food and Nutrition, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung City 43301, Taiwan
Tuzz-Ying Song: Department of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, 168, Xuefu Road, Dacun Township, Changhua City 515006, Taiwan

Agriculture, 2023, vol. 13, issue 1, 1-18

Abstract: Rice protein is a suitable alternative protein source for dairy protein in infant formulas on account of its unique nutrition and hypoallergenicity. Rice protein was isolated through enzymatic hydrolysis (Alcalase, papain, bromelain, Flavourzyme ® ) in combination with high-pressure processing (HPP) (400 MPa for 15 min at 25 °C) to enhance its functional properties and broaden its food processing applications. The effect of the HPP-treated rice protein hydrolysate on dexamethasone (DEX)-induced atrophy in C2C12 myotubes was also studied. The length of myotubes was observed under a light microscope, and periodic acid Schiff staining. The results showed that even though enzymatic hydrolysis and HPP treatment affected the color of the resulting rice protein, the protein content (3120.31 ± 42.15), branched chain amino acid (BCAA) content (15.12 ± 1.03), peptide content (31.25 ± 0.55), and amino acid composition of the rice protein were significantly increased. Moreover, the combined enzymatic and HPP treatment effectively overcame the problem of limited solubility and water-holding capacity. Rice protein produced through enzymatic and HPP treatment exhibited a higher free radical scavenging activity and oxygen radical absorbance capacity. It also alleviated DEX-induced muscle atrophy in C2C12 myotubes as indicated by the increase in myotube length. In short, the enzymatic and HPP treatment of rice protein not only overcame limitations, but also produced rice protein with high functionality in terms of antioxidant and therapeutic effects on muscle atrophy. The rice protein hydrolysate produced through enzymatic hydrolysis and HPP treatment showed the potential for use as an ingredient for functional foods in the nutraceutical industry.

Keywords: anti-sarcopenia; rice protein; high-pressure processing; C2C12 cell atrophy model; periodic acid Schiff staining (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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