Characterization and Fungicide Screening of a New Pathogen That Causes Leaf Spot on Rehmannia glutinosa

Tao Dou, Yubiao Cai, Xuhong Song, Futao Gao, Yajun Zhao, Jiafang Du, Fengqing Wang, Xuanzhen Li, Shiheng An, Xinming Yin, Xiangyang Liu () and Zhongyi Zhang
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Tao Dou: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Yubiao Cai: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Xuhong Song: Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China
Futao Gao: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Yajun Zhao: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Jiafang Du: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Fengqing Wang: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Xuanzhen Li: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Shiheng An: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Xinming Yin: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Xiangyang Liu: Wenhua Road Campus, Henan Agricultural University, Zhengzhou 450002, China
Zhongyi Zhang: College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Agriculture, 2023, vol. 13, issue 2, 1-14

Abstract: Outbreaks of leaf spot disease occurred in Rehmannia glutinosa fields in Henan Province, China, in 2019, with the incidence ranging from 20% to 40%. R. glutinosa plants with diseased leaves were collected, and 25 isolates were obtained. Pathogenicity tests, morphological observations, and phylogenetic analyses were conducted to identify the pathogens, and the biological characteristics and control agents of the pathogens were studied. Five isolates of pathogenic fungi were isolated. Three isolates were identified as Fusarium equiseti , which is a new pathogen causing R. glutinosa leaf disease; the other two isolates were identified as Fusarium acuminatum . The mycelia of F. equiseti grew fastest on Czapek medium, and the optimal temperature and pH were 25 °C and 10.0, respectively. The mycelia of F. equiseti grew from 5 °C t o 35 °C, and the lethal temperature was 55 °C. The optimal carbon and nitrogen sources were soluble starch and peptone, respectively. Eight fungicides had inhibitory effects on the mycelial growth of F. equiseti and F. acuminatum . Prochloraz had higher activities against F. equiseti and F. acuminatum , with EC 50 values of 0.139 mg·L −1 and 0.123 mg·L −1 , respectively. These results provide useful information that will aid the development of management strategies to control leaf diseases of R. glutinosa caused by F. equiseti and F. acuminatum.

Keywords: Rehmannia glutinosa; disease; control; Fusarium equiseti; biological characteristics; antifungal activity (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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