Cold Tolerance Mechanisms in Mungbean ( Vigna radiata L.) Genotypes during Germination

Lekshmi S. Manasa, Madhusmita Panigrahy, Kishore Chandra Panigrahi, Gayatri Mishra, Sanjib Kumar Panda () and Gyana Ranjan Rout ()
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Lekshmi S. Manasa: National Institute of Science, Education and Research, Homi Bhabha National Institute (HBNI), Jatni 752050, India
Madhusmita Panigrahy: National Institute of Science, Education and Research, Homi Bhabha National Institute (HBNI), Jatni 752050, India
Kishore Chandra Panigrahi: National Institute of Science, Education and Research, Homi Bhabha National Institute (HBNI), Jatni 752050, India
Gayatri Mishra: National Institute of Science, Education and Research, Homi Bhabha National Institute (HBNI), Jatni 752050, India
Sanjib Kumar Panda: Department of Biochemistry, Central University of Rajasthan, Ajmer 305817, India
Gyana Ranjan Rout: Department of Agricultural Biotechnology, Odisha University of Agriculture & Technology, Bhubaneswar 751003, India

Agriculture, 2023, vol. 13, issue 2, 1-24

Abstract: Mungbean or greengram ( Vigna radiata ) is an important legume crop well known for its high protein with nitrogen-fixing abilities. However, the severe yield loss in mungbean occurs due to susceptibility to low temperatures at all stages of plant growth including germination and is a serious concern for its cultivation and productivity. To select cold-tolerant genotypes, a germination-based screening at 10 °C was performed in a total of 204 germplasms. The study showed that cold stress of the initial 8-days during seedling establishment imposed a negative impact throughout the life of mungbean genotypes, which were reflected in the vegetative and reproductive phase (plant height, days to 50% flowering and pods/plant, seeds/pod, yield/plant, and 100-seed weight). The biplot analysis showed that parameters such as germination rate index, Timson’s index, mean germination time, and coefficient of the velocity of germination are the key influential germination parameters for identifying cold tolerance in the seedling stage. Identified cold-tolerant genotype (PAU911) retained higher rootlet number, leaf area, and increased chlorophyll, carotenoid, and malondialdehyde (MDA) content at 10 °C. Based on the confocal microscopic study, it is noticed that the stomatal density, open pore percentage, and trichome density were significant differences in seedlings exposed to cold stress as compared to non-stress. On the basis of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis, it is observed that a new protein identified as TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE1 ( TTL1 ) (UNIPROT Identifier: LOC106762419) which highly correlated with the cold stress response of in the cold-tolerant genotype. Our study identifies a noble member, TTL1, whose expression has a positive role in cold tolerance response at the protein level in V. radiata . This study will help breeding programs with regard to the sustainable growth of mungbean.

Keywords: cold stress; greengram; mungbean; seed germination; screening; Vigna radiata; TTL1 gene expression (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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