Backcrossing with Marker Assistance to Introduce Broad-Spectrum Bacterial Leaf Blight Resistance in the Malaysian Elite Variety MR297

Saba Jasim Aljumaili, Mohd Y. Rafii (), Siti Zaharah Sakimin, Khairulmazmi Ahmad and Abdulmajeed Al-Jumaily
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Saba Jasim Aljumaili: Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia
Mohd Y. Rafii: Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia
Siti Zaharah Sakimin: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia
Khairulmazmi Ahmad: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang 43400, Malaysia
Abdulmajeed Al-Jumaily: Department of Signal Theory and Communications, Universidad Carlos III de Madrid, Leganes, 28911 Madrid, Spain

Agriculture, 2023, vol. 13, issue 2, 1-16

Abstract: A significant part of marker-assisted backcross breeding (MABB) is recurrent parent genome recovery (RPGR). The purpose of this study was to introduce MABB-mediated resistance genes against numerous bacterial leaf blight (BLB) pathogens. Here, we examine the introgression of four Xoo resistance genes to the high-yielding Malaysian MR297 lineage Xa21, xa13 , xa5 , and Xa4 from the IRBB60 lineage. To accomplish both foreground and background selection, we employed polymorphic functional linked markers to target genes and simple sequence repeat (SSR) markers. We used 83 of the 475 authorized polymorphic microsatellite markers to determine the proportion of RPGR in the chosen lines. According to the data, the RPGR at BC 1 F 1 , BC 2 F 1 , and BC 2 F 2 are 81.94, 92.30, and 95.04%, respectively. Incorporating the four BLB resistance genes into the newly created lines would result in long-lasting and comprehensive protection against BLB. Therefore, a resistant gene may be introduced into a population more quickly with MABB than through conventional breeding. All ten lines tested showed a significant level of resistance to BLB, with three lines displaying very high levels of resistance. Consequently, it was recommended that new lines should be used to produce commercially viable rice varieties.

Keywords: rice; backcrossing; bacterial leaf blight; foreground and background selection; microsatellites; recurrent parent genome recovery (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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