Exploring Plant Tissue Culture and Steviol Glycosides Production in Stevia rebaudiana (Bert.) Bertoni: A Review

Shilpa Sharma, Swati Gupta, Deepa Kumari, Shanker Lal Kothari, Rohit Jain and Sumita Kachhwaha ()
Additional contact information
Shilpa Sharma: Department of Botany, University of Rajasthan, Jaipur 302004, India
Swati Gupta: Department of Biosciences, Manipal University Jaipur, Jaipur 303007, India
Deepa Kumari: Department of Botany, University of Rajasthan, Jaipur 302004, India
Shanker Lal Kothari: Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur 303002, India
Rohit Jain: Department of Biosciences, Manipal University Jaipur, Jaipur 303007, India
Sumita Kachhwaha: Department of Botany, University of Rajasthan, Jaipur 302004, India

Agriculture, 2023, vol. 13, issue 2, 1-30

Abstract: Stevia rebaudiana (Bert.) Bertoni, commonly called “sweet leaf” is a medicinally and industrially important plant known to be rich in zero-calorie natural sweetening compound(s) known as “steviol glycosides”. However, due to its poor seed germination and slow vegetative propagation, it has become rather difficult to meet the increasing global demand for Stevia -based products. Different biotechnological approaches have been developed over the past few decades to overcome these limitations and allow for mass propagation of the plant. Several protocols for in vitro organogenesis, callogenesis, and cell suspension cultures have been developed during the past few years. Apart from this, attempts have also been made to improve the production of steviol glycosides through nutrient manipulation, the use of elicitors, hairy root cultures, genetic transformation, and metabolic pathway engineering. Therefore, this review provides an up-to-date report on the applications of various biotechnological tools for mass propagation and enhanced steviol glycoside production, along with a detailed bibliometrics analysis. This review also highlights research gaps and future considerations that could be fruitful for the scientific community to delve deeper into the various unexplored aspects of the architecture and functionality of this natural sweetening plant.

Keywords: sweet leaf; bibliometric analysis; stevioside; pathway engineering; in vitro propagation (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/2077-0472/13/2/475/pdf (application/pdf)
https://www.mdpi.com/2077-0472/13/2/475/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:13:y:2023:i:2:p:475-:d:1071007

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().