Use of Gas Chromatography and SPME Extraction for the Differentiation between Healthy and Paenibacillus larvae Infected Colonies of Bee Brood—Preliminary Research

Bartosz Piechowicz, Aleksandra Kwiatek, Stanisław Sadło, Lech Zaręba, Anna Koziorowska (), Daniela Kloc and Maciej Balawejder
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Bartosz Piechowicz: Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland
Aleksandra Kwiatek: Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland
Stanisław Sadło: Department of Ecotoxicology, Faculty of Biotechnology, University of Rzeszów, Rejtana 16c, 35-959 Rzeszow, Poland
Lech Zaręba: Interdisciplinary Centre for Computational Modelling, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland
Anna Koziorowska: Interdisciplinary Center for Preclinical and Clinical Research, University of Rzeszow, Werynia 2, 36-100 Kolbuszowa, Poland
Daniela Kloc: Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland
Maciej Balawejder: Institute of Agricultural Sciences, College of Natural Sciences, University of Rzeszow, Zelwerowicza 8b, 35-601 Rzeszow, Poland

Agriculture, 2023, vol. 13, issue 2, 1-14

Abstract: Paenibacillus larvae is a deadly pathogen for bee brood, which can lead to the death of entire colonies. The presence of specific volatile organic compounds (VOCs) in the hive may be related to the occurrence of this bacterium in brood. Compositions of those volatile fractions present in healthy brood from control colonies and the brood without symptoms of infection collected from the colonies infected by P. larvae were compared using gas chromatography coupled with mass spectrometry (GC-MS) and solid phase microextraction (SPME). Among the seven compounds detected and quantified, the relative concentrations of 3-carene and limonene significantly differentiated the brood from healthy and infected colonies. Based on the ratio analysis, the samples were differentiated in terms of the number of emitted VOCs.

Keywords: honey bee brood; American foulbrood; solid phase microextraction; volatile organic compounds (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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