Base Editing of EUI1 Improves the Elongation of the Uppermost Internode in Two-Line Male Sterile Rice Lines

Yakun Wang, Shengjia Tang, Naihui Guo, Ruihu An, Zongliang Ren, Shikai Hu, Xiangjin Wei, Guiai Jiao, Lihong Xie, Ling Wang, Ying Chen, Fengli Zhao, Peisong Hu, Zhonghua Sheng and Shaoqing Tang ()
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Yakun Wang: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Shengjia Tang: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Naihui Guo: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Ruihu An: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Zongliang Ren: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Shikai Hu: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Xiangjin Wei: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Guiai Jiao: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Lihong Xie: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Ling Wang: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Ying Chen: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Fengli Zhao: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Peisong Hu: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Zhonghua Sheng: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China
Shaoqing Tang: State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Improvement Centre/China National Rice Research Institute, Hangzhou 310006, China

Agriculture, 2023, vol. 13, issue 3, 1-13

Abstract: The use of male sterile lines (MSLs) of rice is essential for heterosis utilization. However, MSLs have a common defect in the elongation of the uppermost internode, eventually leading to incomplete panicle exsertion, blocking pollination, and reducing the hybrid rice seed yield. Previously, the elongated uppermost internode 1 (EUI1) was identified as an active gibberellin-deactivating enzyme that plays a key role in panicle exsertion from the flag leaf sheath in rice ( Oryza sativa L.). We used an adenine base editor to edit EUI1 and obtained two types of homozygous transgenic plants ( eui1-1 and eui1-2 ). The transcription and translation levels of EUI1 in the two mutants were significantly lower than in the wild type, as was the oxidation activity of EUI1 to active gibberellins (GAs), which also decreased. The contents of the plant hormones GA 1 , GA 3 , and GA 4 in eui1-1 (1.64, 1.55, and 0.92 ng/g) and eui1-2 (0.85, 0.64, and 0.65 ng/g) panicles were significantly higher than the wild type (0.70, 0.57, and 0.42 ng/g). The uppermost internode lengths of the mutant were 26.5 and 23.6 cm, which were significantly longer than that of the wild type (18.0 cm), and the cell lengths of the mutant were 161.10 and 157.19 μm, which were longer than that of the wild type (89.28 μm). Our results indicate that the adenine base editing system could increase the content of endogenous bioactive GAs in young panicles by fine-tuning EUI1 activity, reduce the defect of panicle enclosure in MSLs and increase the yield of hybrid rice seed production.

Keywords: EUI1; gibberellin; base editing; male sterile rice line; elongation of internode (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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