Mitigation of Salinity Stress on Soybean Seedlings Using Indole Acetic Acid-Producing Acinetobacter pittii YNA40

Sang-Mo Kang, Md. Injamum Ul Hoque, Ji-In Woo and In-Jung Lee ()
Additional contact information
Sang-Mo Kang: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
Md. Injamum Ul Hoque: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
Ji-In Woo: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea
In-Jung Lee: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea

Agriculture, 2023, vol. 13, issue 5, 1-15

Abstract: Soybean is an important oil crop with multiple uses. Soybeans can grow in various soil types and climates; however, salt stress reduces their yield. Plant growth-promoting microorganisms are an environmentally benign way to combat stress and boost plant tolerance. In the present study, we have identified plant growth-promoting bacteria that can produce indole acetic acid (IAA) and induce distinct growth characteristics in soybean plants under salt stress. The YNA40 isolate was identified as Acinetobacter pittii through 16S rRNA sequencing and phylogenetic analysis. A pure culture of Acinetobacter pittii YNA40 was subjected to chromatographic and mass spectrometry selected-ion monitoring (GC-MS/SIM) for IAA quantification. The results revealed that the YNA40 bacterial strain showed a significantly higher IAA concentration (473.88 ng/mL) at 4% sodium chloride (NaCl). Moreover, in a salt-stress condition, inoculation with Acinetobacter pittii YNA40 was able to induce increased shoot length (23.48%), shoot weight (24%), root length (2.47%), and root weight (44.82%) compared to the uninoculated control. Therefore, soybean seedlings were inoculated with YNA40 to examine their potential for promoting growth and reprogramming after salt stress. Inoculation with YNA40 isolates mitigated the salt stress and significantly improved the growth of the plant, enhanced the chlorophyll contents, and improved the quantum efficiency of chlorophyll fluorescence, total phenolic content, flavonoid content, the diphenyl-1-picrylhydrazyl (DPPH) activity, and antioxidant activities of soybean plants during and after salt stress. The present research demonstrated that the application of the YNA40 isolate is promising for reducing salt stress in soybean plants and helps plants grow better in a salt-stressed environment.

Keywords: plant growth-promoting bacteria; phytohormone; NaCl; SOD; DPPH (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.mdpi.com/2077-0472/13/5/1021/pdf (application/pdf)
https://www.mdpi.com/2077-0472/13/5/1021/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jagris:v:13:y:2023:i:5:p:1021-:d:1141182

Access Statistics for this article

Agriculture is currently edited by Ms. Leda Xuan

More articles in Agriculture from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().