Analysis of the Biological Activity and Whole Genome Sequencing of Bacillus cereus CDHWZ7 Isolated from the Rhizosphere of Lycium ruthenicum on the Tibetan Plateau

Xue Yang, Yongli Xie (), Youming Qiao, Lan Chen, Tian Wang, Lingling Wu, Junxi Li and Ying Gao
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Xue Yang: College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Yongli Xie: College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Youming Qiao: State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China
Lan Chen: College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Tian Wang: College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Lingling Wu: College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Junxi Li: College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
Ying Gao: College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China

Agriculture, 2023, vol. 13, issue 5, 1-17

Abstract: This study examined the biological activity and genome of Bacillus cereus CDHWZ7 isolated from the root of Lycium ruthenicum in the Dachaidan saline area, Haixi Prefecture, Qinghai Province, China. The results revealed that B. cereus CDHWZ7 exhibited strong inhibition activity against the pathogenic fungi Fusarium graminearum , F. acuminatum , and F. oxysporum . CDHWZ7 also demonstrated cellulose-degrading activity, nitrogen-fixing activity, and the ability to secrete indole-3-acetic acid (IAA) at 55.00 mg∙L −1 . The strain CDHWZ7 can grow at a salt concentration of 3–11%, a pH range of 5–11, and a temperature of 4 °C–18 °C, and shows good salt tolerance, acid and alkaline tolerance, and low-temperature fitness. The genome of strain CDHWZ7 was sequenced using Illumina HiSeq + PacBio, revealing a circular structure of 5,648,783 bp in length, containing two intact plasmids with an average GC content of 35.2%, and a total number of 5672 encoded genes. It contained 106 tRNA genes, 42 rRNA genes, and 134 sRNA genes. A total of 137 genes were annotated as carbohydrases, with a total base length of 3,968,396,297 bp. The numbers of coding sequences assigned to the Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups of Proteins, and Gene Ontology Databases were 4038, 4133, and 2160, respectively. Further analysis of the genome identified genes encoding chitinase activity, cellulases, secondary metabolites, phytohormone production, volatile compounds, nitrogen and phosphate metabolism, and resistance responses to biotic stresses (glycine betaine transporter protein, catalase, superoxide dismutase, low-affinity potassium transporter protein, cold-shock protein, heat-shock protein), as well as genes related to proliferation, stress response, and resistance to pathogenic fungi. Therefore, this study determined that strain CDHWZ7 has several excellent biological traits, such as antagonism to pathogenic fungi, nitrogen-fixation ability, cellulose-degradation ability, and IAA-production ability. The genome sequence of strain CDHWZ7 and several biodefense functional genes were also analyzed, revealing the potential use of strain CDHWZ7 in the development of biological agents.

Keywords: antagonistic activity; Bacillus; cellulose degradation activity; extreme environment; IAA producing activity; stress tolerance (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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