Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway

Bo Wang, Yiheng Wang, Likun Xie, Wancong Yu, Qingkuo Lan, Yong Wang (), Chengbin Chen () and Yong Zhang ()
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Bo Wang: Institute of Germplasm Resources and Biotechnology, State Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin 300071, China
Yiheng Wang: Institute of Germplasm Resources and Biotechnology, State Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin 300071, China
Likun Xie: College of Life Sciences, Nankai University, Tianjin 300071, China
Wancong Yu: Institute of Germplasm Resources and Biotechnology, State Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin 300071, China
Qingkuo Lan: Institute of Germplasm Resources and Biotechnology, State Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin 300071, China
Yong Wang: Institute of Germplasm Resources and Biotechnology, State Key Laboratory of Vegetable Biobreeding, Tianjin Academy of Agricultural Sciences, Tianjin 300071, China
Chengbin Chen: College of Life Sciences, Nankai University, Tianjin 300071, China
Yong Zhang: Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China

Agriculture, 2023, vol. 13, issue 7, 1-13

Abstract: Because rice is native to tropical and subtropical regions, it is generally more sensitive to cold stress compared to other cereals. Low-temperature stress has a strong influence on the growth, development, and yield of rice. Plant NAM, ATAF1/2 and CUC(NAC) transcription factors (TFs) play significant regulatory roles in stress response. In our research, we found that OsNAC050 (LOC_Os03g60080) is mainly expressed in leaves. Cold (4 °C), heat (42 °C), PEG 6000 (20%, w / v ), NaCl (200 mM), H 2 O 2 (1%), IAA (100 μM), ABA (100 μM) and GA3 (100 μM) were used to treat wild type (WT) plants. A low temperature further up-regulated OsNAC050 expression. OsNAC050 mutants created using CRISPR-Cas9 gene editing technology showed significantly enhanced tolerance to the low-temperature treatment. The measurement of enzyme activities related to the redox pathway also showed that mutants have stronger viability under low-temperature stress. Comparative transcriptome analysis showed that photosynthesis and soluble sugar metabolism were significantly affected in OsNAC050 mutant lines, suggesting that OsNAC050 may participate in the above molecular pathways in response to low-temperature stress. The results expand our understanding of the molecular mechanisms underlying the responses to cold stress in rice and can provide new strategies for engineering cold tolerance in high-yielding rice varieties.

Keywords: CRISPR-Cas9; cold stress; RNA-seq; photosynthesis; soluble sugar (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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