Trehalose as a Stabilizer of the Lipid Composition of Membranes and the Composition of the Cytosol of Frozen/Thawed Rooster Spermatozoa

Olga Stanishevskaya, Yulia Silyukova (), Vera Tereshina, Elena Ianutsevich, Nikolai Pleshanov, Anton Kurochkin and Elena Fedorova
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Olga Stanishevskaya: Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, Pushkin, 196625 St. Petersburg, Russia
Yulia Silyukova: Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, Pushkin, 196625 St. Petersburg, Russia
Vera Tereshina: Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia
Elena Ianutsevich: Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia
Nikolai Pleshanov: Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, Pushkin, 196625 St. Petersburg, Russia
Anton Kurochkin: Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, Pushkin, 196625 St. Petersburg, Russia
Elena Fedorova: Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the LK Ernst Federal Research Center for Animal Husbandry, Moskovskoe Shosse, Pushkin, 196625 St. Petersburg, Russia

Agriculture, 2023, vol. 13, issue 7, 1-12

Abstract: Low-temperature semen storage technologies are already being used in poultry conservation programs, but the quality of reproductive material stored in cryobanks varies greatly and cannot always be successfully used for practical purposes. Therefore, it is necessary to improve the compositions of cryoprotective media to improve their quality. This study aimed to investigate the composition of membrane lipids and carbohydrates in the cytosol of rooster spermatozoa, to explain the dose-dependent effect of a combination of trehalose and fructose in cryoprotective media on the preservation of their morphological and kinetic parameters during freezing/thawing, and to determine the most effective diluent composition. Ejaculates were collected from Rhode Island Red roosters (n = 10). The effectiveness of three diluents containing trehalose was evaluated: LCM-control (0 mM), Treh20 (9.5 mM), and Treh30 (13.4 mM). Chromatographic analysis of membrane lipids, carbohydrates, and polyols of the spermatozoa cytosol was performed. A decrease in the content of glycolipids in the plasma membranes of spermatozoa from 2.0% in native spermatozoa to 1.1–1.4% (frozen/thawed) and phospholipids from 71.2% (native) to 70.5% (frozen/thawed) reduced the progressive sperm motility from 65.7% in native spermatozoa to 12.6–27.6% (frozen/thawed). The same dynamics were observed for the viability parameter of 90.4% (native) and 27.0–41.2% (frozen/thawed). The Treh20 diluent, using a combination of fructose (36 mM) and trehalose (9.5 mM) saccharides, maximally preserved the lipid profile of plasma membranes and the composition of the cytosol of frozen/thawed rooster spermatozoa, which positively affected the indicators of general and progressive mobility and viability.

Keywords: cryopreservation; poultry genetic resources; rooster spermatozoa; trehalose; plasma membrane; cytosol; sperm cryopreservation; lipids; carbohydrates (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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