Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent Pseudomonas as Biocontrol Agents for the Management of Soil-Borne Fungi, Fusarium oxysporum and Rhizoctonia solani

Gaber Attia Abo-Zaid (), Ahmed Salah Abdullah, Nadia Abdel-Mohsen Soliman, Ebaa Ebrahim El-Sharouny, Abdulaziz A. Al-Askar, Yiming Su, Ahmed Abdelkhalek () and Soraya Abdel-Fattah Sabry
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Gaber Attia Abo-Zaid: Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, Alex 21934, Egypt
Ahmed Salah Abdullah: Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, Alex 21934, Egypt
Nadia Abdel-Mohsen Soliman: Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, Alex 21934, Egypt
Ebaa Ebrahim El-Sharouny: Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Alex 21526, Egypt
Abdulaziz A. Al-Askar: Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Yiming Su: Utah Water Research Laboratory, Department of Civil and Environmental Engineering, Utah State University, Logan, UT 84341, USA
Ahmed Abdelkhalek: Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt
Soraya Abdel-Fattah Sabry: Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Alex 21526, Egypt

Agriculture, 2023, vol. 13, issue 7, 1-14

Abstract: Secretion of siderophores by Pseudomonas aeruginosa F2 and P . fluorescens JY3 was evaluated on chrome azurol S (CAS) agar plates and their inhibitory effect was inspected against Fusarium oxysporum and Rhizoctonia solani . Production of siderophores as biocontrol agents from F2 and JY3 was accomplished in two optimized media. Afterward, cell-free supernatants of the bacterial cultures containing siderophores were used for the preparation of two bio-friendly formulations for the management of F . oxysporum and R . solani under greenhouse conditions. The investigated bacterial isolates, F2 and JY3, showed antagonistic activity in vitro against F . oxysporum and R . solani and produced siderophores in optimized media with high efficiency. Colonies of both bacterial isolates were grown exponentially with a constant specific growth rate of 0.07 h −1 and 0.27 h −1 , correspondingly. Siderophores estimated in 10 µL reached their highest value of 16.95% at 47 h and 19.5% at 48 h for isolate F2 and JY3, respectively. Formulations of siderophore-generating F2 and JY3 reduced damping-off caused by F . oxysporum by 40% and 80%, while the reduction percentage of damping-off caused by R . solani reached 87.5% and 62.5%, correspondingly. Moreover, both formulations encouraged the growing of wheat plants where the fresh and dry weight of shoots and roots were increased compared to the treatment with each fungus. In conclusion, bio-friendly formulations resulting from this investigation can play an active role in managing soil-borne diseases.

Keywords: siderophores; biocontrol; Pseudomonas aeruginosa; Pseudomonas fluorescens; bioformulation (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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