Genome-Wide Identification and Expression Analysis of the CesA/Csl Gene Superfamily in Alfalfa ( Medicago sativa L.)

Bilig Sod, Lei Xu, Yajiao Liu, Fei He, Yanchao Xu, Mingna Li, Tianhui Yang, Ting Gao, Junmei Kang, Qingchuan Yang and Ruicai Long ()
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Bilig Sod: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Lei Xu: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yajiao Liu: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Fei He: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yanchao Xu: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Mingna Li: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Tianhui Yang: Institute of Animal Sciences, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China
Ting Gao: Institute of Animal Sciences, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China
Junmei Kang: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Qingchuan Yang: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Ruicai Long: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Agriculture, 2023, vol. 13, issue 9, 1-14

Abstract: The cellulose synthase ( CesA ) and cellulose synthase-like ( Csl ) superfamily encodes critical enzymes involved in processing plant cellulose and hemicellulosic polysaccharides. The alfalfa ( Medicago sativa L.) genome was sequenced in recent years, but this superfamily remains poorly understood at the genome-wide level. We identified 37 members of the CesA/Csl family from the alfalfa genome in this study as well as their chromosomal locations and synteny. We uncovered 28 CesA/Csl expressed across all tissues and CslD genes specifically expressed in the root. In addition, cis-acting element analysis showed that CesA/Csl contained several abiotic stress-related elements. Moreover, transcriptomic analysis of alfalfa seedlings demonstrated the involvement of this superfamily in responses to cold, drought, and salt stresses. Specifically, CslD increased expression in cold conditions and decreased under osmotic stress, highlighting its potential role in stress adaptation. The findings offer valuable information for the practical exploration of the functions of CesA/Csl during plant development and the development of enhanced tolerance to different stress conditions.

Keywords: alfalfa; CesA/Csl; cell wall; forage quality; phylogenetic analysis; gene expression (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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