An Efficient Micropropagation Protocol for the Endangered European Shrub February Daphne ( Daphne mezereum L.) and Identification of Bacteria in Culture

Karolina Nowakowska, Potshangbam Nongdam, Nabilah Amany Samsurizal and Andrzej Pacholczak ()
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Karolina Nowakowska: Section of Ornamental Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland
Potshangbam Nongdam: Department of Biotechnology, Manipur University, Canchipur, Imphal 795003, India
Nabilah Amany Samsurizal: Section of Ornamental Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland
Andrzej Pacholczak: Section of Ornamental Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland

Agriculture, 2023, vol. 13, issue 9, 1-13

Abstract: Daphne mezereum of the Thymelaeaceae family is a medicinal shrub occurring naturally in Europe and under legal protection in Poland. In the present study, a protocol developed for mass propagation of February daphne from nodal explants is presented. Micropropagation is one of the in vitro techniques that allow the preservation of rare and valuable plants by developing efficient methods for their propagation. In the proliferation stage, explants were cultured in the Woody Plant Medium (WPM) with different cytokinins, and in the rooting stage on the semi-solid WPM medium with perlite, indole-3-butyric acid (IBA) in various concentrations was used. The maximum proliferation rate (five shoots per explant) was observed on the medium containing 4.14 μM of meta-Topolin (mT). When the regenerated shoots were rooted in vitro in the presence of IBA in various concentrations, that of 19.68 μM induced the highest number of roots per shoot (6.63) and the maximal root length (2.15 cm). It is also worth remembering that plants are often colonized by different groups of microorganisms, which also affect the diversity of the ecosystem. The endophytic bacteria inhabiting the D. mezereum shoots are Mycobacterium .

Keywords: in vitro; medicinal plant; endophytic bacteria; plant growth regulators (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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