Unraveling the Function of Stress Kinase in the Progeny of Soybean Plants Grown from Low-Temperature Pretreated Seeds

Mariana Radkova, Miglena Revalska and Anelia Iantcheva ()
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Mariana Radkova: Agrobioinstitute, Agricultural Academy, Blvd. Dragan Tsankov 8, 1164 Sofia, Bulgaria
Miglena Revalska: Agrobioinstitute, Agricultural Academy, Blvd. Dragan Tsankov 8, 1164 Sofia, Bulgaria
Anelia Iantcheva: Agrobioinstitute, Agricultural Academy, Blvd. Dragan Tsankov 8, 1164 Sofia, Bulgaria

Agriculture, 2024, vol. 14, issue 10, 1-15

Abstract: The research work was carried out with the progeny of two soybean cultivars, Richy and Izidor, from the years 2019 and 2020. Plants were grown from seeds pretreated with low temperature (2–5 °C) before sowing for two periods of treatment: 12 days marked as “treated control” (tr. K) and 22 days marked as “treated” (tr.); and “non-treated” (K0) used as a control. Transcriptional profiles of the gene encoding a stress protein kinase were evaluated after the application of abiotic stresses caused by the following: 150 mM NaCl solution/salinity stress/for 24 h; 350 mM mannitol solution/drought stress/for 24 h; and low temperature (4 °C) for 72 h. Transcript levels were established by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) in leaf tissue collected from seedlings of the 2019 and 2020 progeny of “tr. K”, “tr.”, and “K0” samples. Analyses determining the quantity of malondialdehyde (MDA) and total antioxidant capacity (TAC) were performed. The expression of investigated stress kinase was highly upregulated after the application of abiotic stress caused by 150 mM solution of NaCl and to a lesser extent by 350 mM solution of mannitol. Detected transcript levels depend on the type of sample out of “tr. K”, “tr.”, and “K0”; the progeny; and the genotype.

Keywords: soybean; stress protein kinase; abiotic stress; expression profiles; antioxidant analyses (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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