Effects of OsLPR2 Gene Knockout on Rice Growth, Development, and Salt Stress Tolerance

Ying Gu, Chengfeng Fu, Miao Zhang, Changqiang Jin, Yuqi Li, Xingyu Chen, Ruining Li, Tingting Feng, Xianzhong Huang () and Hao Ai ()
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Ying Gu: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Chengfeng Fu: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Miao Zhang: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Changqiang Jin: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Yuqi Li: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Xingyu Chen: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Ruining Li: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Tingting Feng: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Xianzhong Huang: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China
Hao Ai: Center for Crop Biotechnology, Anhui Science and Technology University, Chuzhou 239000, China

Agriculture, 2024, vol. 14, issue 10, 1-12

Abstract: Rice ( Oryza sativa L.), a globally staple food crop, frequently encounters growth, developmental, and yield limitations due to phosphate deficiency. LOW PHOSPHATE ROOT1 / 2 ( LPR1 / 2 ) are essential genes in plants that regulate primary root growth and respond to local phosphate deficiency signals under low phosphate stress. In rice, five LPR genes, designated OsLPR1 – OsLPR5 based on their sequence identity with AtLPR1 , have been identified. OsLPR3 and OsLPR5 are specifically expressed in roots and induced by phosphate deficiency, contributing to rice growth, development, and the maintenance of phosphorus homeostasis under low phosphate stress. In contrast, OsLPR2 is uniquely expressed in shoots, suggesting it may have distinct functions compared with other family members. This study employed Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) gene editing technology to generate oslpr2 mutant transgenic lines and subsequently investigated the effect of OsLPR2 gene knockout on rice growth, phosphate utilization, and salt stress tolerance in the seedling stage, as well as the effect of OsLPR2 gene knockout on rice development and agronomic traits in the maturation stage. The results indicated that the knockout of OsLPR2 did not significantly impact rice seedling growth or phosphate utilization, which contrasts significantly with its homologous genes, OsLPR3 and OsLPR5 . However, the mutation influenced various agronomic traits at maturity, including plant height, tiller number, and seed setting rate. Moreover, the OsLPR2 mutation conferred enhanced salt stress tolerance in rice. These findings underscore the distinct roles of OsLPR2 compared with other homologous genes, establishing a foundation for further investigation into the function of the OsLPR family and the functional differentiation among its members.

Keywords: rice; OsLPR2; phosphate; growth and development; salt tolerance (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2024
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